CD4 Antikörper (CD4 Molecule) (AA 420-457)

Details for Product anti-CD4 Antibody No. ABIN671376, Anbieter: Anmelden zum Anzeigen
Antigen
  • cd4
  • L3T4
  • Ly-4
  • CD4mut
  • W3/25
  • p55
  • CD4 molecule
  • CD4 antigen
  • T-cell surface glycoprotein CD4
  • Cd4 molecule
  • CD4
  • cd4
  • Cd4
Epitop
AA 420-457
66
64
21
19
15
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14
13
12
10
9
8
8
8
8
6
6
5
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5
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3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
1219
631
233
68
67
40
36
27
26
22
21
19
18
16
15
15
13
10
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5
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4
3
3
3
3
2
2
2
2
2
1
1
1
1
Wirt
Kaninchen
1377
587
280
11
4
1
1
1
Klonalität
Polyklonal
Konjugat
Dieser CD4 Antikörper ist unkonjugiert
266
227
170
103
49
48
46
40
36
34
34
30
27
25
21
21
19
19
18
18
18
18
16
15
15
13
13
13
11
9
9
8
6
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5
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3
3
3
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2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Applikation
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
1744
490
444
353
323
309
156
155
88
78
38
38
31
30
30
25
18
14
14
11
9
9
6
6
5
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
'Independent Validation' Siegel
Chargennummer 130703
Validierte Anwendung Immunohistochemistry
Positivkontrolle Mouse spleen tissue
Negativkontrolle Mouse muscle tissue
Bewertung Signal was detected in positive control sample and not in negative control sample.
Primärantikörper
Isotypkontrolle
Sekundärantikörper
Kontrollen
  • Positive control: Spleen tissue (specimen known to contain the target protein) from mouse.
  • Negative Control: Muslce tissue (specimen known to not contain the target protein) from mouse.
  • Primary antibody isotype control: Mouse spleen treated with primary antibody isotype control instead of the primary antibody.
  • Secondary antibody only control: Mouse spleen treated with secondary antibody only (no primary antibody).
Protokoll
  • Immunohistochemistry was performed manually.
  • Sections were deparaffinized and rehydrated.
  • Sections were heated to 98°C for 10 min in 0.1M Borate buffer with 1 mM NaCl and 1 mM EDTA pH 8.0 for antigen retrieval.
  • Sections were incubated in 3% hydrogen peroxide for 10 min at room temperature to block endogenous peroxidases.
  • Sections were washed x 3 in Tris buffered saline (TBS).
  • Sections were blocked in 5 % normal donkey serum for 60 min at room temperature.
  • Sections were incubated with primary antibody diluted 1:200 in 5% normal donkey serum in TBS. Incubated at 4°C temperature overnight.
  • Sections were washed x 3 in Tris buffered saline.
  • Sections were incubated with secondary antibody diluted 1:400 in 5% normal donkey serum in TBS. Incubated for 60 min at room temperature..
  • Sections were washed x 3 in Tris buffered saline.
  • Sections were incubated with Vectastain ABC kit (Vector Lab PK6100) to enhance signal.
  • Sections were incubated with DAB chromogenic substrate (Vector Lab SK-4105) for 60 sec at RT.
  • Sections were washed x 3 in Distilled Water.
  • Sections were counterstained with hematoxylin for 20 sec.
  • Sections were washed x 1 in Distilled Water.
  • Sections were dehydrated, mounted and photographed under a Zeiss AxioImager M1 light microscope.
Anmerkungen - No experimental challenges noted.
Validierungsbilder
Immunohistochemistry validation image for anti-CD4 Molecule (CD4) (AA 420-457) antibody (ABIN671376) Figure 1: Mouse spleen stained with anti-CD4 (brown) and counterstained with hematoxy...
Immunohistochemistry validation image for anti-CD4 Molecule (CD4) (AA 420-457) antibody (ABIN671376) Figure 2: Mouse muscle stained with anti-CD4 (brown) and counterstained with hematoxy...
Immunohistochemistry validation image for anti-CD4 Molecule (CD4) (AA 420-457) antibody (ABIN671376) Figure 3: Mouse spleen stained with isotype control antibody (brown) and counterstain...
Immunohistochemistry validation image for anti-CD4 Molecule (CD4) (AA 420-457) antibody (ABIN671376) Figure 4: Mouse spleen stained with secondary antibody only (brown) and counterstaine...
Immunogen KLH conjugated synthetic peptide derived from rat CD4
Isotyp IgG
Reinigung Purified by Protein A.
Andere Bezeichnung CD4 (CD4 Antibody Abstract)
Hintergrund

Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation.

Subcellular location: Cytoplasm

Synonyms: p55, W3/25, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, W3/25 antigen, CD4

Gen-ID 24932
UniProt P05540
Forschungsgebiet Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways T-Zell Rezeptor Signalweg, Maintenance of Protein Location, CXCR4-mediated Signaling Events
Applikationshinweise WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20°C
Haltbarkeit 12 months
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-CD4 Molecule (CD4) (AA 420-457) antibody (ABIN671376) Formalin-fixed and paraffin embedded human colon carcinoma labeled Anti-CD4 Polyclona...
Immunofluorescence (IF) image for anti-CD4 Molecule (CD4) (AA 420-457) antibody (ABIN671376) Formalin-fixed and paraffin-embedded rat lung labeled with Anti-CD4 Polyclonal Antibo...
Immunohistochemistry (IHC) image for anti-CD4 Molecule (CD4) (AA 420-457) antibody (ABIN671376) Independently Validated Antibody, image provided by Science Direct, badge number 0296...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-CD4 Molecule (CD4) (AA 420-457) antibody (ABIN671376) Image was kindly submitted by Dr.David M Burmeister from US Army Institute of Surgica...
Flow Cytometry (FACS) image for anti-CD4 Molecule (CD4) (AA 420-457) antibody (ABIN671376) Mouse spleen cells probed with Rabbit Anti-CD4 Polyclonal Antibody, PE Conjugated fo...
Western Blotting (WB) image for anti-CD4 Molecule (CD4) (AA 420-457) antibody (ABIN671376) Lane 1: CD4 recombinant protein Lane 2: Raji cell lysate probed with at 1:5000 90min...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-CD4 Molecule (CD4) (AA 420-457) antibody (ABIN671376) Paraformaldehyde-fixed, paraffin embedded rat kidney, Antigen retrieval by boiling in...
Produkt verwendet in: Smit, Bester, van den Heever, Schlegel, Botes, Dohmen: "Does prolonged post-mortem cold ischemic harvesting time influence cryopreserved pulmonary homograft tissue integrity?" in: Cell and tissue banking, 2015 (PubMed).