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CD4 Antikörper (AA 385-457)

CD4 Reaktivität: Human, Maus, Ratte, Huhn, Schwein FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN671376
  • Target Alle CD4 Antikörper anzeigen
    CD4
    Bindungsspezifität
    • 31
    • 16
    • 16
    • 16
    • 15
    • 9
    • 7
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 385-457
    Reaktivität
    • 378
    • 221
    • 119
    • 37
    • 23
    • 22
    • 20
    • 19
    • 15
    • 11
    • 10
    • 10
    • 8
    • 7
    • 7
    • 6
    • 6
    • 6
    • 6
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Maus, Ratte, Huhn, Schwein
    Wirt
    • 361
    • 145
    • 132
    • 4
    • 2
    • 1
    • 1
    Kaninchen
    Klonalität
    • 502
    • 137
    • 3
    Polyklonal
    Konjugat
    • 202
    • 69
    • 57
    • 51
    • 32
    • 15
    • 14
    • 14
    • 12
    • 11
    • 10
    • 9
    • 9
    • 8
    • 7
    • 7
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    Dieser CD4 Antikörper ist unkonjugiert
    Applikation
    • 509
    • 204
    • 113
    • 109
    • 85
    • 79
    • 78
    • 41
    • 39
    • 38
    • 32
    • 25
    • 17
    • 10
    • 9
    • 8
    • 8
    • 7
    • 6
    • 6
    • 6
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))
    Kreuzreaktivität
    Huhn, Human, Maus, Schwein, Ratte
    Homologie
    Dog,Cow,Sheep,Guinea Pig
    Aufreinigung
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic peptide derived from Human CD4 (cytoplasmic domain)
    Isotyp
    IgG
  • Applikationshinweise
    WB 1:300-5000
    ELISA 1:500-1000
    FCM 1:20-100
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Validierung #029634 (Immunohistochemistry)
    'Independent Validation' Siegel
    by
    Confocal Imaging Core, Beth Israel Deaconess Medical Center
    No.
    #029634
    Datum
    26.03.2014
    Antigen
    Chargennummer
    130703
    Validierte Anwendung
    Immunohistochemistry
    Positivkontrolle
    Mouse spleen tissue
    Negativkontrolle
    Mouse muscle tissue
    Bewertung
    Signal was detected in positive control sample and not in negative control sample.
    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    • Antibody: CD4 Molecule (CD4)
    • Catalog number: ABIN671376
    • Lot number: 130703
    Sekundärantikörper
    • Antibody: Donkey anti-Rabbit IgG Antibody (Biotinylated)
    Full Protocol
    • Immunohistochemistry was performed manually.
    • Sections were deparaffinized and rehydrated.
    • Sections were heated to 98°C for 10 min in 0.1M Borate buffer with 1 mM NaCl and 1 mM EDTA pH 8.0 for antigen retrieval.
    • Sections were incubated in 3% hydrogen peroxide for 10 min at room temperature to block endogenous peroxidases.
    • Sections were washed x 3 in Tris buffered saline (TBS).
    • Sections were blocked in 5 % normal donkey serum for 60 min at room temperature.
    • Sections were incubated with primary antibody diluted 1:200 in 5% normal donkey serum in TBS. Incubated at 4°C temperature overnight.
    • Sections were washed x 3 in Tris buffered saline.
    • Sections were incubated with secondary antibody diluted 1:400 in 5% normal donkey serum in TBS. Incubated for 60 min at room temperature..
    • Sections were washed x 3 in Tris buffered saline.
    • Sections were incubated with Vectastain ABC kit (Vector Lab PK6100) to enhance signal.
    • Sections were incubated with DAB chromogenic substrate (Vector Lab SK-4105) for 60 sec at RT.
    • Sections were washed x 3 in Distilled Water.
    • Sections were counterstained with hematoxylin for 20 sec.
    • Sections were washed x 1 in Distilled Water.
    • Sections were dehydrated, mounted and photographed under a Zeiss AxioImager M1 light microscope.
    Anmerkungen
    - No experimental challenges noted.
  • Format
    Liquid
    Konzentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    Konservierungsmittel
    ProClin
    Vorsichtsmaßnahmen
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Haltbarkeit
    12 months
  • Jaime-Ramirez, Yu, Caserta, Yoo, Zhang, Lee, Hofmeister, Lee, Kumar, Pan, Kumar, Baiocchi, Teknos, Pichiorri, Kaur, Old: "Reolysin and Histone Deacetylase Inhibition in the Treatment of Head and Neck Squamous Cell Carcinoma." in: Molecular therapy oncolytics, Vol. 5, pp. 87-96, (2017) (PubMed).

    Zhang, Fu, Li, Wang, Zhang, Liu: "Bone morphogenetic protein 7 is associated with the nodal invasion of colon cancer." in: Oncology letters, Vol. 11, Issue 3, pp. 1707-1712, (2016) (PubMed).

    Smit, Bester, van den Heever, Schlegel, Botes, Dohmen: "Does prolonged post-mortem cold ischemic harvesting time influence cryopreserved pulmonary homograft tissue integrity?" in: Cell and tissue banking, (2015) (PubMed).

  • Target
    CD4
    Andere Bezeichnung
    CD4 (CD4 Produkte)
    Synonyme
    cd4 antikoerper, L3T4 antikoerper, Ly-4 antikoerper, CD4mut antikoerper, W3/25 antikoerper, p55 antikoerper, CD4 molecule antikoerper, carcinoembryonic antigen-related cell adhesion molecule 5 antikoerper, T-cell surface glycoprotein CD4 antikoerper, CD4 antigen antikoerper, Cd4 molecule antikoerper, CD4 antikoerper, LOC100125525 antikoerper, cd4 antikoerper, Cd4 antikoerper
    Hintergrund

    Synonyms: T-cell surface glycoprotein CD4, Cd4, T-cell surface antigen T4/Leu-3, W3/25 antigen

    Background: Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.

    Gen-ID
    24932
    UniProt
    P01730
    Pathways
    T-Zell Rezeptor Signalweg, Maintenance of Protein Location, CXCR4-mediated Signaling Events
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