MNDA Antikörper (FITC)

Antigen: MNDA

Klonalität: Monoklonal (3C1)

Wirt: Ratte

Konjugat: FITC

Applikation: Durchflusszytometrie (FACS), Fluoreszenzmikroskopie (FM), In situ-Hybridisierung (ISH)

Produktnummer: ABIN648953

Produktbeschreibung

Isotyp: IgG1 kappa

Klon: 3C1

Beschreibung: Antigen distribution: The antibody reacts to the human myeloid nuclear differentiation antigen (MNDA). Cross reactivity to a similar protein in other species has not been extensively characterized. Most applications will require permeabilization or solubilization of cell membranes, as the primary cell localization of this protein is intranuclear [1]. MNDA has been characterized as a cofactor in regulation of cell cycle progression, cell differentiation and apoptosis [2-4]. The human myeloid nuclear differentiation antigen, MNDA, is expressed only in myelomonocytic and a subset of B lymphoid hematopoietic cells [5]. MNDA is uniformly distributed throughout the interphase cell nucleus and associates with chromatin, but does not bind specific DNA sequences [6]. It is a member of the interferon-alpha inducible protein family and accordingly has been reported to be altered in a variety of inflammatory related disease states [3, 7- 11]. Expression is disproportionately consistent in the marginal zone lymphoproliferative disorders subset of malignant lymphoma [12]. Expression is reported to be decreased in myelodysplasia in myeloid precursors and mature forms [13, 14].45-15 is suitable for intracellular staining of cytospots and frozen tissue sections by immunohistochemistry. Cellular Expression: nuclear protein expressed by myeloid cells and a subset of B-cells.

Spezifität: Concentrated nuclear protein extract prepared from late stage human myeloid leukemia HL-60 cells..45-15 produces mouse IgG1 immunoglobulins directed against human IFN-γ [1-4]. It is generally advisable to use a specificity control such as one of the following: 1) unlabeled antibody, 2) recombinant cytokines or 3) mouse isotype control

Anwendungen

Forschungsgebiet: Stammzellen, Hämopoetische Vorläufer, Angeborene Immunität, Zytokine

Beschränkungen: Nur für Forschungszwecke einsetzbar