Anthrax Protective Antigen (PA) Antikörper

Antigen: Anthrax Protective Antigen (PA)

Wirt: Maus

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN639882

Produktbeschreibung

Produktmerkmale: Mouse Anti-Anthrax Protective Antigen

Weitere Bezeichnung: Anthrax Protective Antigen

Immunogen: Highly purified protective antigen of Bacillus anthracis.

Isotyp: IgG2b

Spezifität: DS-MB-02603 recognizes the Protective Antigen of the anthrax toxin. Bacillus anthracis produces a toxin composed of three proteins, the Protective Antigen (PA), the Edema Factor (EF) and the Lethal Factor (LF). The three proteins depend on each other for their toxic effect. Both EF and LF functions intracellularly but are too large to enter the cell through existing channels. The PA is responsible for transporting the EF and LF into cells. The 83kDa PA (PA83) binds to the cell surface where a 20 kDa N-terminal fragment (PA20) is cleaved off, exposing binding sites for LF and EF. The remaining 63 kDa portion (PA63) oligomerizes to form a heptamer pre-pore, which then associates with up to three molecules of EF and/or LF. The receptor complex is internalized within endocytotic vessels that are subsequently acidified, transforming the PA heptamer into a pore that releases the active enzymes EF (an adenylate cyclase) and LF (a protease targeting MAPKKs) into the cytosol. DS-MB-02603 does not cross-react with the Lethal Factor of Bacillus anthracis, Yersinia pestis, Francisella tularensis or Toxoplasma gondii. The antibody recognizes both the 83kDa and 63kDa truncated form of PA.

Anwendungen

Konzentration: IgG concentration 1.0 mg/ml

Reinigung: Purified

Buffer: Phosphate buffered saline pH7.4

Konservierungsmittel: 0.09% Sodium Azide (NaN 3 )

Lagerung: Store at +4 ° C or at -20 ° C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of shipment

Forschungsgebiet: Virologie

Beschränkungen: Nur für Forschungszwecke einsetzbar