Histone H1 Antikörper
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| Synonyme |
H1c, H1.2, H1f2, His1a, H1var1, MGC107646, 0610008C09Rik, LOC692942, HISTONE H1, HON4 |
| Klonalität |
Monoklonal (AE-4) |
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Wirt
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Reaktivität
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Applikation
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 Alternativen Western Blot (WB), ELISA, Immunfluoreszenz (IF), Immunhistochemie (Paraffinschnitte) (IHC (p)), Immunhistochemie (Gefrierschnitte) (IHC (fro))
Immunfluoreszenz (IF) (39), Western Blot (WB) (34), Durchflusszytometrie (FACS) (18), ELISA (16), Immunhistochemie (Paraffinschnitte) (IHC (p)) (15), Immunpräzipitation (IP) (11), Immunhistochemie (IHC) (10), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (7), Enzyme Immunoassay (EIA) (4), Immunzytochemie (ICC) (4), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Chromatin-Immunopräzipitation (ChiP) (1), Immunoelectron Microscopy (IEM) (1)
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| Produktnummer |
ABIN573964 |
| Menge |
50 µg (1 mg/ml) |
| Preis |
493,59 € Zzgl. Versandkosten €20,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 10 bis 15 Werktagen |
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Immunogen
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Cells, Nuclei from myeloid leukemia biopsy cells.
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Isotyp
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IgG2a (Passende Sekundärantikörper)
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Klon
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AE-4
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Beschreibung
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Gene: Histone H1
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Spezifität
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Recognizes histone H1. The histone proteins serve as a structural scaffold for the organization of nuclear DNA into chromatin. In eukaryotic cells there are 4 core histone proteins, H2A, H2B, H3 and H4 that assemble to form an octamer round which the DNA is wrapped in a sequence dependant manner. The resulting complex is known as a nucleosome and is the basic subunit of chromatin. Histone H1 acts as a linker and binds to each nucleosome, and by self affinity, links these nucleosomes together resulting in the 30 nm chromatin fiber. In blots of rat brain homogenate a duplet is observed at 27-29 kD consistent with Histone H1. Speckled staining is observed in the nucleus of normal and malignant cells.
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Applikationshinweise
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ELISA, IF, IHC-Fr, IHC-P (5 µg/ml), WB (1:100)
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Konzentration
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1 mg/ml
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Reinigung
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Protein G Column
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Buffer
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PBS, 0.09% sodium azide.
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Lagerung
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+4°C or -20°C, Avoid repeated freezing and thawing.
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Forschungsgebiet
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Chromatin und nukleäre Signaltransduktion, DNS/RNS, Transkriptionsfaktoren
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Alternativen zu Antigen "Histone H1", Typ "Antikörper" finden
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Wirte
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Kaninchen (29), Maus (20), Schaf (6), Huhn (5)
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Reaktivitäten
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Human (46), Maus (22), Rind (Kuh) (21), Ratte (Rattus) (18), Hund (16), Pferd (16), Kaninchen (16), Schwein (15), Säugetiere (1)
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Applikationen
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Immunfluoreszenz (IF) (39), Western Blot (WB) (34), Durchflusszytometrie (FACS) (18), ELISA (16), Immunhistochemie (Paraffinschnitte) (IHC (p)) (15), Immunpräzipitation (IP) (11), Immunhistochemie (IHC) (10), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (7), Enzyme Immunoassay (EIA) (4), Immunzytochemie (ICC) (4), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Chromatin-Immunopräzipitation (ChiP) (1), Immunoelectron Microscopy (IEM) (1)
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Konjugate
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitope
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Acetyl-Lys25 (1), pThr17 (1)
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