TRPC7 Antikörper (Internal Region)

Produktnummer ABIN570738

Produktdetails anti-TRPC7 Antikörper

Target: TRPC7 (Transient Receptor Potential Cation Channel, Subfamily C, Member 7 (TRPC7))

Bindungsspezifität: Internal Region

Reaktivität: Maus, Huhn

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser TRPC7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Verwendungszweck: TRPC7

Sequenz: KLCKSKAKSC END

Spezifität: This antibody is expected to cross-react with isoform 1, 2 and 3 (NP_065122.1, NP_001161049.1, NP_001161048.1).

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse: Verified

Immunogen: Peptide with sequence KLCKSKAKSCEND, from the internal region of the protein sequence according to NP_065122.1, NP_001161049.1, NP_001161048.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: A 95 kDa band was observed in Chicken Embryo Heart atrial and ventricular myocyte lysates (Sabourin et al, PMID 21672930). This antibody has been successfully used in WB on Mouse (Murtaza et al., PMID: 32882106).

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunoprecipitation: This antibody has been successfully used in IP on lysates of Chicken Embryo Heart (Sabourin et al, PMID: 21672930).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-TRPC7 Antikörper (ABIN570738)

Sabourin, Antigny, Robin, Frieden, Raddatz: "Activation of transient receptor potential canonical 3 (TRPC3)-mediated Ca2+ entry by A1 adenosine receptor in cardiomyocytes disturbs atrioventricular conduction." in: The Journal of biological chemistry, Vol. 287, Issue 32, pp. 26688-701, (2012) (PubMed).

Sabourin, Robin, Raddatz: "A key role of TRPC channels in the regulation of electromechanical activity of the developing heart." in: Cardiovascular research, Vol. 92, Issue 2, pp. 226-36, (2011) (PubMed).

Details zu TRPC7

Target: TRPC7 (Transient Receptor Potential Cation Channel, Subfamily C, Member 7 (TRPC7))

Andere Bezeichnung: TRPC7 (TRPC7 Produkte)

Synonyme: TRP7 antikoerper, TRP-7 antikoerper, Trrp8 antikoerper, TRPC7 antikoerper, TRPM2 antikoerper, transient receptor potential cation channel subfamily C member 7 antikoerper, transient receptor potential cation channel, subfamily C, member 7 antikoerper, short transient receptor potential channel 7 antikoerper, TRPC7 antikoerper, Trpc7 antikoerper, LOC100012798 antikoerper

Hintergrund: TRPC7, transient receptor potential cation channel, subfamily C, member 7, TRP7, likley ortholog of mouse transient receptor potential cation channel, subfamily C, member 7, putative capacitative calcium channel

Gen-ID: 57113, 26946, 282822

NCBI Accession: NP_065122, NP_001161049, NP_001161048