Flavivirus Group Antigen Antikörper

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Antigen
Reaktivität
Flavivirus
15
1
1
Wirt
Mammalian Cells
15
2
Antikörpertyp
Recombinant Antibody
Klonalität (Klon)
Monoklonal ()
Konjugat
Unkonjugiert
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
ELISA, Immunofluorescence (IF), Neutralization (Neut), Western Blotting (WB)
16
15
14
2
2
2
Optionen
Hersteller
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Verwendungszweck Monoclonal antibody to detect Flavivirus group antigen.
Immunogen Dengue Virus type 2 antigens
Klon D1-4G2-4-15 (4G2)
Isotyp IgG2a, kappa
Reinigung Affinity purified using protein A
Hintergrund This antibody binds to flavivirus group antigen, protein E. It can be used as an anti-Dengue virus antibody, anti-West Nile virus antibody, anti-Japanese Encephalitis or anti-Zika Virus antibody (Aubry et al. 2016) to identify cells infected with these flaviviridae. It binds to the fusion loop at the extremity of domain II of E protein from all four serotypes and prevents syncytia formation. The epitope is highly conserved amongst flaviviridae and has been functionally analyzed in detail by Crill and Chang 2004. Previous studies have used acetone- (Henchal et al. 1982) or methanol-fixed slides (Moreland & Tay, 2010). Please note that binding of this antibody has been reported to be sensitive to reduction and Western Blots should be performed under non-reducing conditions (Lai et al. 2008).
Synonyms: N/A
Applikationshinweise Optimal working dilution should be determined by the investigator.
Kommentare

Suggested positive control:
ELISA: Dengue Virus Serotype 2 VLPs

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer 200 μg affinity purified antibody in phosphate-buffered saline (PBS) containing 0.02 % Proclin 300
Konservierungsmittel ProClin
Vorsichtsmaßnahmen This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung The antibody solution should be gently mixed before use
Lagerung -20 °C
Haltbarkeit 12 months
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