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His Tag Antikörper

Details zu Produkt Nr. ABIN559683, Anbieter: Anmelden zum Anzeigen Neu
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Synonyme AKR1B11, AKR1B12, ALDRLn, ARL-1, ARL1, HIS, HSI
Reaktivität
Tag
(486), (23), (7), (3)
Wirt
Maus
(276), (181), (32), (26), (3)
Klonalität (Klon)
Monoklonal ()
Konjugat
Unkonjugiert
(51), (39), (37), (15), (12), (9), (4), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation
Dot Blot (DB), Immunostaining (ISt), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
(436), (279), (168), (76), (65), (35), (33), (13), (13), (11), (8), (6), (5), (4), (4), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 7 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen Neu
Produkt-ID vom Hersteller Anmelden zum Anzeigen Neu
Menge 100 μg
Lieferung nach Deutschland ( )
Verfügbarkeit Lieferung in 5 bis 7 Werktagen
Immunogen HHHHHH (6x His) synthetic peptide conjugated to KLH
Sequenz HHHHHH
Klon HIS-H8
Isotyp IgG2b
Spezifität Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags)
Sensitivität WB (with ECL): 1-2ng of His-tagged fusion proteins
Reinigung Protein A affinity chromatography from mouse ascites fluid
Substanzklasse Tag
Forschungsgebiet Tags/Labels
Pathways
Applikationshinweise WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature) / IS: 1:500-2000 dilution / For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 1mg/mL
Buffer 10mM PBS, pH 7.2, 0.05% NaN3
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Bilder des Herstellers
 image for anti-His Tag Antikörper (ABIN559683) LEFT: untransfected control, RIGHT: anti-His (in red) on His-tagged fusion proteins i...
 image for anti-His Tag Antikörper (ABIN559683) Comparison between anti-His tag (HIS.H8 / EH158) mAb with 2 different vendor Abs, pro...
 image for anti-His Tag Antikörper (ABIN559683) Standard ladder containing five different His-tagged proteins, untransfected control ...
Produkt verwendet in: Chi, Bo, Wu et al.: "Vimentin and PSF act in concert to regulate IbeA+ E. coli K1 induced activation and nuclear translocation of NF-κB in human brain endothelial cells." in: PLoS ONE, Vol. 7, Issue 4, pp. e35862, 2012 (PubMed).

Hu, Benedict, Ding et al.: "Role of cytochrome c and dATP/ATP hydrolysis in Apaf-1-mediated caspase-9 activation and apoptosis." in: The EMBO journal, Vol. 18, Issue 13, pp. 3586-95, 1999 (PubMed).

Green, Reed: "Mitochondria and apoptosis." in: Science (New York, N.Y.), Vol. 281, Issue 5381, pp. 1309-12, 1998 (PubMed).

Srinivasula, Ahmad, Fernandes-Alnemri et al.: "Autoactivation of procaspase-9 by Apaf-1-mediated oligomerization." in: Molecular cell, Vol. 1, Issue 7, pp. 949-57, 1998 (PubMed).

Allgemeine Veröffentlichungen Cecconi, Alvarez-Bolado, Meyer et al.: "Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development." in: Cell, Vol. 94, Issue 6, pp. 727-37, 1998 (PubMed).

Li, Nijhawan, Budihardjo et al.: "Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade." in: Cell, Vol. 91, Issue 4, pp. 479-89, 1997 (PubMed).

Zou, Henzel, Liu et al.: "Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3." in: Cell, Vol. 90, Issue 3, pp. 405-13, 1997 (PubMed).

Produktnummer ABIN559683
160,89 €
Zzgl. Versandkosten 20,00 € und MWSt

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