Dieser Intestinal Alkaline Phosphatase Antikörper ist unkonjugiert
Applikation
Western Blotting (WB), Enzyme Immunoassay (EIA)
Spezifität
This antibody detects human Alkaline phosphatase. Cross reactivity against Alkaline phosphatase from other tissues and species may occur but have not been specifically determined. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Alkaline phosphatase [human intestine].
Aufreinigung
Prepared from monospecific antiserum by a delipidation and defibrination.
ALPI
Reaktivität: Human
Wirt: Kaninchen
Polyclonal
FITC
Applikationshinweise
Suitable for Immunoblotting (Western or Dot blot), ELISA and most immunological methodsrequiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of AlkalinePhosphatase [Human Intestine] in a standard sandwich ELISA using Peroxidase conjugatedAffinity Purified anti-Rabbit IgG [H&L] (Goat) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 5,000 to 1: 20,000 of the reconstitutionconcentration is suggested for this product. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Rekonstitution
Restore with 2.0 mL of deionized water (or equivalent).
Konzentration
85.0 mg/mL (by Refractometry)
Buffer
0.02 M Potassiumphosphate, 0.15 M Sodium chloride, pH 7.2 with 0.01 % (w/v) Sodium azide as preservative.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Store vial at -20 °C prior to opening. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20 °C or below.