s100a4 Antikörper (C-Term)

Produktnummer ABIN375119

Produktdetails anti-s100a4 Antikörper

Target: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser s100a4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequenz: CNEFFEGFPD KQP

Spezifität: This antibody recognizes S100A4 / CAPL.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog).
Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Synthetic Peptide from the C-Terminus of Human S100A4 (NP_002952.1 and NP_062427.1) Genename: S100A4

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit Dilution: 1/128000. Western blot: 0.03-0.1 μg/mL. Approx 13 kDa band observed in Human Placenta lysates. Immunohistochemistry on Paraffin Sections: 2-3 μg/mL. In paraffin embedded Human Small Intestine shows strong nuclear staining in some cellsand weaker cytoplasm staining in some other cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu s100a4

Target: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Andere Bezeichnung: S100A4 (S100A4 Produkte)

Synonyme: 18A2 antikoerper, 42A antikoerper, CAPL antikoerper, FSP1 antikoerper, MTS1 antikoerper, P9KA antikoerper, PEL98 antikoerper, 42a antikoerper, Capl antikoerper, FSp1 antikoerper, Mts1 antikoerper, PeL98 antikoerper, metastasin antikoerper, pk9a antikoerper, S100A4 antikoerper, P9ka antikoerper, RNP9KA antikoerper, S100 calcium binding protein A4 antikoerper, S100 calcium-binding protein A4 antikoerper, S100A4 antikoerper, S100a4 antikoerper

Hintergrund: S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. S100 protein is also expressed in the antigen presenting cells such as the Langerhans cells in skin and interdigitating reticulum cells in the paracortex of lymph nodes. S100A4 has been implicated in motility, invasion and tubulin polymerization. It is also involved in tumor metastasis.Synonyms: CAPL, Calvasculin, FSP-1, FSP1, Fibroblast marker, Fibroblast specific protein-1, MTS1, Metastasin, Placental Calcium-Binding Protein, S100 Calcium-Binding Protein A4, S100-A4

Molekulargewicht: 11.7 kDa (NP_002952.1 and NP_062427.1).

Gen-ID: 6275, 9606

UniProt: P26447

Pathways: S100 Proteine