KCNJ6 Antikörper (C-Term)

Produktnummer ABIN374875

Produktdetails anti-KCNJ6 Antikörper

Target: KCNJ6 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 6 (KCNJ6))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser KCNJ6 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Sequenz: C-SSKLNQHAEL ET

Spezifität: This antibody detects KCNJ6.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, RatSpecies reactivity (tested):Human

Aufreinigung: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide from the C Terminus of the protein sequence according to NP_002231.1 Genename: KCNJ6

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: limit dilution 1: 16000. Western blot: 1-3 μg/mL. Approx 48 kDa band observed in Human Brain (Hippocampus andSubstantia Nigra) lysates (calculated MW of 48.8 kDa according to NP_002231.1). Anadditional band of unknown identity was also consistently observed at 38 kDa. This bandwas successfully blocked by incubation with the immunising peptide. We would appreciateany feedback from people in the field - have any such results been reported with otherantibodies/lysates? Have any further splice variants/modified forms been reported?
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Details zu KCNJ6

Target: KCNJ6 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 6 (KCNJ6))

Andere Bezeichnung: KCNJ6 (KCNJ6 Produkte)

Synonyme: kcnj6 antikoerper, BIR1 antikoerper, GIRK-2 antikoerper, GIRK2 antikoerper, KATP-2 antikoerper, KATP2 antikoerper, KCNJ7 antikoerper, KIR3.2 antikoerper, hiGIRK2 antikoerper, Kir3.2 antikoerper, weaver antikoerper, wv antikoerper, potassium voltage-gated channel subfamily J member 6 antikoerper, potassium channel, inwardly rectifying subfamily J, member 5 antikoerper, potassium inwardly-rectifying channel, subfamily J, member 6 antikoerper, KCNJ6 antikoerper, kcnj5 antikoerper, Kcnj6 antikoerper

Hintergrund: G-protein regulated inward-rectifier potassium channels (GIRK) are part of a superfamily of inward-rectifier K+ channels which includes seven family members. To date four GIRK subunits, designated GIRK1-4 (also designated Kir3.1-4), have been identified in mammals, and GIRK5 has been found in Xenopus oocytes. GIRK channels exist in vivo both as homotetramers and heterotetramers. GIRK channels are modulated by G-proteins, they are also modulated by phosphatidylinositol 4,5-bisphosphate, intracellular sodium, ethanol and mechanical stretch. GIRK1, 2 and 3 are highly abundant in brain. In general, neuronal GIRK channels are involved in the regulation of the excitability of neurons and may contribute to the resting potential.Synonyms: BIR1, G protein-activated inward rectifier potassium channel 2, GIRK2, Inward rectifier K(+) channel Kir3.2, KATP-2, KATP2, KCNJ6, KCNJ7, Potassium channel, inwardly rectifying subfamily J member 6

Gen-ID: 3763

NCBI Accession: NP_002231

UniProt: P48051