ADH5 Antikörper (Internal Region)

Produktnummer ABIN374869

Produktdetails anti-ADH5 Antikörper

Target: ADH5 (Alcohol Dehydrogenase 5 (Class III), chi Polypeptide (ADH5))

Bindungsspezifität: Internal Region

Reaktivität: Human, Maus, Ratte, Schwein, Rind (Kuh), Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ADH5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequenz: C-KKIKVDEFVT HN

Spezifität: This antibody detects Alcohol Dehydrogenase 5 (ADH5).

Kreuzreaktivität (Details): Species reactivity (expected):Canine (Dog), Pig, Bovine.
Species reactivity (tested):Human, Mouse, Rat.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_000662.3 Genename: ADH5

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1: 1000. Western blot: 1-3 μg/mL. Customer's experiments gave a band at approx 38 kDa in MouseLymphocyte lysates (calculated MW of 39.8 kDa according to Human NP_000662.3 and39.8 kDa according to Mouse NP_031436.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu ADH5

Target: ADH5 (Alcohol Dehydrogenase 5 (Class III), chi Polypeptide (ADH5))

Andere Bezeichnung: Alcohol Dehydrogenase 5 (ADH5) (ADH5 Produkte)

Synonyme: ADH2 antikoerper, ADH-3 antikoerper, ADHX antikoerper, FALDH antikoerper, FDH antikoerper, GSH-FDH antikoerper, GSNOR antikoerper, ADH1C antikoerper, ADH3 antikoerper, wu:fb60b11 antikoerper, Adh-5 antikoerper, Adh3 antikoerper, adh-3 antikoerper, adh3 antikoerper, adh5 antikoerper, adhx antikoerper, fdh antikoerper, gsnor antikoerper, ADH4 antikoerper, ADH5 antikoerper, alcohol dehydrogenase 1B (class I), beta polypeptide antikoerper, alcohol dehydrogenase 5 (class III), chi polypeptide antikoerper, alcohol dehydrogenase 5 antikoerper, alcohol dehydrogenase 5 (class III), chi polypeptide L homeolog antikoerper, alcohol dehydrogenase class-3 antikoerper, ADH1B antikoerper, ADH5 antikoerper, adh5 antikoerper, Adh5 antikoerper, adh5.L antikoerper, LOC101112223 antikoerper

Hintergrund: The ADH5 gene encodes glutathione dependent formaldehyde dehydrogenase or class III alcohol dehydrogenase chi subunit, which is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, retinol, ethanol and other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class III alcohol dehydrogenase is a homodimer composed of 2 chi subunits. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long chain primary alcohols and for oxidation of S hydroxymethyl glutathione, a spontaneous adduct between formaldehyde and glutathione. The enzyme is an important component of cellular metabolism for the elimination of formaldehyde, which is a potent irritant and sensitizing agent that causes rhinitis, lacrymation, contact dermatitis and pharyngitis.Synonyms: Alcohol dehydrogenase class 3 chi chain, Glutathione-dependent formaldehyde dehydrogenase, S-(hydroxymethyl)glutathione dehydrogenase

Molekulargewicht: 39.7 kDa according to NP_000662.3.

Gen-ID: 128, 9606

UniProt: P11766