Adaptor Protein, phosphotyrosine Interaction, PH Domain and Leucine Zipper Containing 1 (APPL1) (Internal Region) Antikörper

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Antigen
  • APPL
  • DIP13alpha
  • 2900057D21Rik
  • 7330406P05Rik
  • AI585782
  • AW209077
  • BB022931
  • C88264
  • DIP13
  • RGD1309388
  • adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1
  • APPL1
  • Appl1
Epitop
Internal Region
13
9
5
5
4
3
3
3
2
2
1
1
1
1
1
1
1
1
Reaktivität
Rind (Kuh), Hund, Human, Maus, Ratte (Rattus)
67
32
21
6
4
3
3
3
3
3
1
1
Wirt
Ziege
47
19
3
Klonalität
Polyklonal
Konjugat
Unkonjugiert
1
1
1
1
1
1
Applikation
Western Blotting (WB)
63
35
18
12
3
3
1
1
1
Optionen
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Immunogen Peptide with sequence from the internal region of the protein sequence according to NP_036228.1.
Sequenz C-NRYSRLSKKR ENDK
Spezifität This antibody recognizes APPL1.
Kreuzreaktivität (Details) Species reactivity (expected):Mouse, Rat, Canine, Bovine.
Species reactivity (tested):Human.
Reinigung Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
Andere Bezeichnung APPL1 / DIP13A (APPL1 Antibody Abstract)
Hintergrund APPL is involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The APPL binds to RAB5A/Rab5 through N-terminal domain. This interaction is essential for its recruitment to endosomal membranes as well as its role in cell proliferation.Synonyms: Adapter protein containing PH domain, DCC-interacting protein 13-alpha, Dip13-alpha, KIAA1428, PTB domain and leucine zipper motif 1
Gen-ID 26060, 9606
UniProt Q9UKG1
Applikationshinweise Peptide ELISA: 1/64000. Western Blot: 0.3-1 μg/mL. Approx 80 kDa band observed in Rat Aortic Smooth SkeletalMuscle Cells (lane 1), intensity increased after infection with Human APPL1 adenovirus for48h (lane 2). Calculated MW of 79.0 kDa according to NP_036228.1. The blot wasco-labeled with Tubulin-specific antibodies as a loading control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 0.5 mg/mL
Buffer Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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