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MLF1IP Antikörper (Thr78)

MLF1IP Reaktivität: Human, Rind (Kuh), Maus, Hund, Ratte, Schimpanse WB, EIA Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN401336
  • Target Alle MLF1IP Antikörper anzeigen
    MLF1IP (MLF1 Interacting Protein (MLF1IP))
    Bindungsspezifität
    • 15
    • 8
    • 8
    • 6
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Thr78
    Reaktivität
    • 45
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    Human, Rind (Kuh), Maus, Hund, Ratte, Schimpanse
    Wirt
    • 45
    Kaninchen
    Klonalität
    • 45
    Polyklonal
    Konjugat
    • 21
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser MLF1IP Antikörper ist unkonjugiert
    Applikation
    • 43
    • 23
    • 14
    • 14
    • 8
    • 5
    • 4
    • 4
    • 3
    • 2
    • 1
    Western Blotting (WB), Enzyme Immunoassay (EIA)
    Spezifität
    This antibody is directed against MLF1IP protein.
    Kreuzreaktivität (Details)
    Species reactivity (expected):Canine, Bovine, Chimpanzee, Rat, Mouse.
    Species reactivity (tested):Human.
    Aufreinigung
    Affinity chromatography
    Immunogen
    Synthetic peptide corresponding to amino acids surrounding Thr78 of human MLF1IP protein
    Isotyp
    IgG
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  • Applikationshinweise
    ELISA: 1/5,000 - 1/25,000. Western Blot: 1/500- 1/2,000.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1.06 mg/mL (by UV absorbance at 280 nm)
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Az
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    -20 °C
  • Target
    MLF1IP (MLF1 Interacting Protein (MLF1IP))
    Andere Bezeichnung
    MLF1IP (MLF1IP Produkte)
    Synonyme
    MGC147270 antikoerper, CENP50 antikoerper, CENPU antikoerper, CENPU50 antikoerper, KLIP1 antikoerper, PBIP1 antikoerper, CENP-U antikoerper, CENP-50 antikoerper, 1700029A22Rik antikoerper, centromere protein U antikoerper, MLF1 interacting protein antikoerper, cenpu antikoerper, MLF1IP antikoerper, CENPU antikoerper, Cenpu antikoerper
    Hintergrund
    Myeloid leukemia factor-1 (MLF1) Interacting Protein (also known as PBIP1, MLF1IP1, KLIP1 or KSHV latent nuclear antigen interacting protein 1) is a novel polo-like kinase 1 (Plk1) substrate. Plk1 phosphorylation of MLF1IP induces ubiquitination and degradation of MLF1IP prior to the metaphase/anaphase transition. Several Plk1-dependent phosphorylation sites have been identified on MLF1IP by mass spectrometry. Mutations of these sites stabilize MLF1IP and inhibit mitotic progression. Subsequent in vitro and in vivo MLF1IP phosphorylation and stability assays have revealed that phosphorylation of Thr78 is critical for triggering Plk1- dependent MLF1IP degradation. Expression of a non-degradable Thr78Ala mutant was sufficient to induce a mitotic block. Timely phosphorylation of MLF1IP on Thr78 by Plk1 is critical for eliminating the MLF1IP-imposed mitotic block prior to anaphase onset. MLF1IP is speculated to be a novel tumor suppressor, whose function is required for proper sister-chromatid separation. Loss of MLF1IP function may result in improper segregation of chromosomes and genomic instability, thus promoting tumorigenesis.Synonyms: CENP-U(50), CENPU, Centromere protein U, ICEN24, Interphase centromere complex protein 24, KLIP1, KSHV latent nuclear antigen-interacting protein 1, MLF1-interacting protein, PBIP, Polo-box-interacting protein 1
    Gen-ID
    79682
    NCBI Accession
    NP_078905
    UniProt
    Q71F23
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