UBA1 Antikörper

Produktnummer ABIN401028

Produktdetails anti-UBA1 Antikörper

Target: UBA1 (Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1))

Reaktivität: Human, Ratte, Maus, Hund, Kaninchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser UBA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Spezifität: This antibody is directed against Ubiquitin Activating Enzyme E1 protein.

Kreuzreaktivität (Details): Species reactivity (expected):Rabbit, Mouse, Rat, Canine.
Species reactivity (tested):Human.

Aufreinigung: Purified

Immunogen: Recombinant protein corresponding to full length Human Ubiquitin Activating Enzyme E1 fused with GST

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/2,000 - 1/10,000. Western Blot: 1/1,000 - 1/5,000. Immunohistochemistry: 2 μg/mL - 20 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Reconstitute with 0.1 mL of deionized water or equivalent.

Konzentration: 5.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu UBA1

Target: UBA1 (Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1))

Andere Bezeichnung: UBE1 / UBA1 (UBA1 Produkte)

Synonyme: A1S9 antikoerper, A1S9T antikoerper, A1ST antikoerper, AMCX1 antikoerper, GXP1 antikoerper, POC20 antikoerper, SMAX2 antikoerper, UBA1A antikoerper, UBE1 antikoerper, UBE1X antikoerper, DDBDRAFT_0190927 antikoerper, DDBDRAFT_0220497 antikoerper, DDB_0190927 antikoerper, DDB_0220497 antikoerper, Sbx antikoerper, Ube-1 antikoerper, Ube1x antikoerper, a1s9 antikoerper, a1s9t antikoerper, a1st antikoerper, amcx1 antikoerper, gxp1 antikoerper, poc20 antikoerper, smax2 antikoerper, uba1a antikoerper, ube1 antikoerper, ube1x antikoerper, uba1 antikoerper, uba1b antikoerper, ube antikoerper, zgc:66143 antikoerper, wu:fa01e08 antikoerper, wu:fb30f01 antikoerper, wu:fi21c11 antikoerper, wu:fj14g11 antikoerper, ubiquitin like modifier activating enzyme 1 antikoerper, ubiquitin activating enzyme E1 antikoerper, Ubiquitin-conjugating enzyme E1 antikoerper, ubiquitin-like modifier activating enzyme 1 antikoerper, ubiquitin-like modifier activating enzyme 1 S homeolog antikoerper, ubiquitin-like modifier activating enzyme 1 L homeolog antikoerper, UBA1 antikoerper, uae1 antikoerper, GL50803_4083 antikoerper, GL50803_10661 antikoerper, Uba1 antikoerper, uba1.S antikoerper, uba1.L antikoerper, uba1 antikoerper, ptr3 antikoerper

Hintergrund: Ubiquitin Activating Enzyme (E1), also known as A1S9 and UBE1, is responsible for the first step in ubiquitinprotein isopeptide bond formation. E1 catalyzes the activation of the C-terminal carboxyl group of ubiquitin by forming a highenergy thioester bond in an ATP-dependent manner. UBE1 is monomeric and contains two active sites within the E1 Molecule, allowing it to bind two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site. Alternative splicing results in 2 transcript variants encoding the same protein, but with different 5' UTR. Isoform 1 has a different 5' noncoding exon compared to isoform 2. Both variants encode the same protein.Synonyms: A1S9T, Protein A1S9, Ubiquitin-activating enzyme E1, Ubiquitin-like modifier-activating enzyme 1

Gen-ID: 7317

NCBI Accession: NP_003325

UniProt: P22314