MDM2 Antikörper (C-Term)

Produktnummer ABIN5518668

Produktdetails anti-MDM2 Antikörper

Target: MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

Bindungsspezifität: AA 343-358, C-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MDM2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Verwendungszweck: Rabbit IgG polyclonal antibody for E3 ubiquitin-protein ligase Mdm2(MDM2) detection. Tested with WB in Human,Mouse,Rat.

Sequenz: EKAKLENSTQ AEEGFD

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for E3 ubiquitin-protein ligase Mdm2(MDM2) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: MDM2 oncogene, E3 ubiquitin protein ligase
Protein Name: E3 ubiquitin-protein ligase Mdm2

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human MDM2(343-358aa EKAKLENSTQAEEGFD), different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide, Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Referenzen für anti-MDM2 Antikörper (ABIN5518668)

Xiao, Zhou, Yi, Luo, Jiang, Tian, Li, Xue: "Fra-1 is downregulated in cervical cancer tissues and promotes cervical cancer cell apoptosis by p53 signaling pathway in vitro." in: International journal of oncology, Vol. 46, Issue 4, pp. 1677-84, (2015) (PubMed).

Li, Liu, Wang, Fang, Shen, Cai, Gao, Li, Zhang, Ye: "The oncoprotein HBXIP modulates the feedback loop of MDM2/p53 to enhance the growth of breast cancer." in: The Journal of biological chemistry, Vol. 290, Issue 37, pp. 22649-61, (2015) (PubMed).

Liao, Xiao, Zhu, Zheng, He, Pei, Li, Zhou: "CD38 is highly expressed and affects the PI3K/Akt signaling pathway in cervical cancer." in: Oncology reports, Vol. 32, Issue 6, pp. 2703-9, (2014) (PubMed).

Details zu MDM2

Target: MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

Andere Bezeichnung: MDM2 (MDM2 Produkte)

Synonyme: ACTFS antikoerper, HDMX antikoerper, hdm2 antikoerper, zgc:109834 antikoerper, 1700007J15Rik antikoerper, AA415488 antikoerper, Mdm-2 antikoerper, MDM2 proto-oncogene antikoerper, MDM2 proto-oncogene S homeolog antikoerper, Mdm2, p53 E3 ubiquitin protein ligase homolog (mouse) antikoerper, MDM2 oncogene, E3 ubiquitin protein ligase antikoerper, transformed mouse 3T3 cell double minute 2 antikoerper, MDM2 antikoerper, mdm2.S antikoerper, Mdm2 antikoerper, mdm2 antikoerper

Hintergrund: Mdm2 is an important negative regulator of the p53 tumor suppressor. It is the name of a gene as well as the protein encoded by that gene. Mdm2 protein functions both as an E3 ubiquity lipase that recognizes the N-terminal trans-activation domain(TAD) of the p53 tumor suppressor and an inhibitor of p53 transcriptional activation. Oliner et al.(1992) used MDM2 clones to localize the human gene to chromosome 12q13-q14 by analysis of human-hamster somatic cell hybrids.

Synonyms: Double minute 2 protein antibody|E3 ubiquitin-protein ligase Mdm2 antibody|Hdm 2 antibody|HDM2 antibody|MDM 2 antibody|MDM2 antibody|Mdm2 transformed 3T3 cell double minute 2 p53 binding protein(mouse) binding protein 104 kDa antibody|MDM2_HUMAN antibody|MDM2BP antibody|Mouse Double Minute 2 antibody|MTBP antibody|Murine Double Minute Chromosome 2 antibody|Oncoprotein Mdm2 antibody|p53 Binding Protein Mdm2 antibody|p53-binding protein Mdm2 antibody|Ubiquitin protein ligase E3 Mdm2 antibody

UniProt: Q00987

Pathways: p53 Signalweg, PI3K-Akt Signalweg, Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Autophagie, Ubiquitin Proteasome Pathway