Optimal working dilution should be determined by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Buffer
In 1x PBS, pH 7.4
Handhabung
Aliquot to avoid repeated freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Edgar, Manna, Nishimura, Banting, Robinson: "Tetherin is an exosomal tether." in: eLife, Vol. 5, (2016) (PubMed).
Brinkmann, Nehlmeier, Walendy-Gnirß, Nehls, González Hernández, Hoffmann, Qiu, Takada, Schindler, Pöhlmann: "The Tetherin Antagonism of the Ebola Virus Glycoprotein Requires an Intact Receptor-Binding Domain and Can Be Blocked by GP1-Specific Antibodies." in: Journal of virology, Vol. 90, Issue 24, pp. 11075-11086, (2016) (PubMed).
Yang, Lopez, Exline, Haworth, Cannon: "Lack of adaptation to human tetherin in HIV-1 group O and P." in: Retrovirology, Vol. 8, pp. 78, (2011) (PubMed).
Gupta, Mlcochova, Pelchen-Matthews, Petit, Mattiuzzo, Pillay, Takeuchi, Marsh, Towers: "Simian immunodeficiency virus envelope glycoprotein counteracts tetherin/BST-2/CD317 by intracellular sequestration." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 49, pp. 20889-94, (2010) (PubMed).
Hauser, Lopez, Yang, Oldenburg, Exline, Guatelli, Cannon: "HIV-1 Vpu and HIV-2 Env counteract BST-2/tetherin by sequestration in a perinuclear compartment." in: Retrovirology, Vol. 7, pp. 51, (2010) (PubMed).
Karetnikov, Suomalainen: "Tethered virions are intermediates in the assembly and release of HIV-1 particles." in: Virology, Vol. 407, Issue 2, pp. 289-95, (2010) (PubMed).
Lopez, Yang, Hauser, Exline, Haworth, Oldenburg, Cannon: "Ebola virus glycoprotein counteracts BST-2/Tetherin restriction in a sequence-independent manner that does not require tetherin surface removal." in: Journal of virology, Vol. 84, Issue 14, pp. 7243-55, (2010) (PubMed).
Iwabu, Fujita, Kinomoto, Kaneko, Ishizaka, Tanaka, Sata, Tokunaga: "HIV-1 accessory protein Vpu internalizes cell-surface BST-2/tetherin through transmembrane interactions leading to lysosomes." in: The Journal of biological chemistry, Vol. 284, Issue 50, pp. 35060-72, (2009) (PubMed).