PUMA Antikörper (N-Term)

Produktnummer ABIN500559

Produktdetails anti-PUMA Antikörper

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Bindungsspezifität: N-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PUMA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Spezifität: This antibody detects PUMA at N-term.

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Peptide Affinity Chromatography

Immunogen: Synthetic peptide corresponding to 14 amino acids near the amino terminus of Human PUMA-alpha. .Remarks: This sequence is identical between alpha and beta forms of the PUMA proteins.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA. Western blot: 2 to 4 μg/mL, K562 cell lysate can be used as a positive control and a band atapproximately 23 kDa can be detected. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS, 0.02 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu PUMA

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Andere Bezeichnung: PUMA (BBC3 Produkte)

Synonyme: BBC3 antikoerper, Puma antikoerper, JFY-1 antikoerper, JFY1 antikoerper, PUMA antikoerper, PUMA/JFY1 antikoerper, BCL2 binding component 3 antikoerper, Bcl-2 binding component 3 antikoerper, BBC3 antikoerper, Bbc3 antikoerper, bbc3 antikoerper

Hintergrund: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.Synonyms: BBC3, Bcl-2-binding component 3, JFY-1, p53 up-regulated modulator of apoptosis

Gen-ID: 27113

NCBI Accession: NP_055232

UniProt: Q9BXH1

Pathways: p53 Signalweg, Positive Regulation of Endopeptidase Activity