PUMA Antikörper (C-Term)
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- Target Alle PUMA (BBC3) Antikörper anzeigen
- PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
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Bindungsspezifität
- C-Term
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Reaktivität
- Human, Maus
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser PUMA Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)
- Spezifität
- This antibody detects PUMA at C-term.
- Kreuzreaktivität (Details)
- Species reactivity (tested):Human, Mouse.
- Aufreinigung
- Peptide Affinity Chromatography
- Immunogen
- Synthetic peptide corresponding to 14 amino acids near the carboxy terminus of Human PUMA alpha. .Remarks: The sequence is identical between alpha and beta forms of the PUMA proteins.
- Isotyp
- IgG
- Top Product
- Discover our top product BBC3 Primärantikörper
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- Applikationshinweise
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ELISA. Western blot: 1 - 2 μg/mL. K562 or 3T3 cell lysate can be used as a positive control and aband at approximately 23 kDa can be detected. A lower band at approximately 16 kDa wasdetected in MOLT4 and U937 cells, which may represent the PUMA-b form. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Buffer
- PBS, 0.02 % Sodium Azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Avoid repeated freezing and thawing.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
- Andere Bezeichnung
- PUMA (BBC3 Produkte)
- Synonyme
- BBC3 antikoerper, Puma antikoerper, JFY-1 antikoerper, JFY1 antikoerper, PUMA antikoerper, PUMA/JFY1 antikoerper, BCL2 binding component 3 antikoerper, Bcl-2 binding component 3 antikoerper, BBC3 antikoerper, Bbc3 antikoerper, bbc3 antikoerper
- Hintergrund
- Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.Synonyms: BBC3, Bcl-2-binding component 3, JFY-1, p53 up-regulated modulator of apoptosis
- Molekulargewicht
- 23 kDa
- Gen-ID
- 27113
- NCBI Accession
- NP_055232
- UniProt
- Q9BXH1
- Pathways
- p53 Signalweg, Positive Regulation of Endopeptidase Activity
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