ZNF148 Antikörper

Produktnummer ABIN498436

Produktdetails anti-ZNF148 Antikörper

Target: ZNF148 (Zinc Finger Protein 148 (ZNF148))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ZNF148 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: This antibody detects endogenous levels of ZBP-89 protein. (region surrounding Glu84)

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography

Reinheit: > 95 % by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu ZNF148

Target: ZNF148 (Zinc Finger Protein 148 (ZNF148))

Andere Bezeichnung: ZNF148 (ZNF148 Produkte)

Synonyme: BERF-1 antikoerper, BFCOL1 antikoerper, HT-BETA antikoerper, ZBP-89 antikoerper, ZFP148 antikoerper, pHZ-52 antikoerper, 2210405J08Rik antikoerper, AI480666 antikoerper, AW045217 antikoerper, Znf148 antikoerper, Zbp-89 antikoerper, fb06h05 antikoerper, wu:fb06h05 antikoerper, zfp149 antikoerper, zgc:165342 antikoerper, SLC12A8 antikoerper, zinc finger protein 148 antikoerper, ZNF148 antikoerper, Zfp148 antikoerper, znf148 antikoerper

Hintergrund: ZBP-89, also known as BFCOL1, BERF1 and ZNF 148, is a zinc finger transcription factor that is universally expressed. ZBP-89, a Kruppel-like repressor protein, is the silencer element binding factor for Vimentin. ZBP-89 has been shown to bind to GC-rich DNA elements in promoters for gastrin, ornithine decarboxylase and the cyclin-dependent kinase inhibitor p21 (also designated Cip1 or WAF1). ZBP-89 expression is induced by trans-retinoic acid or butyrate, which also induces terminal differentiation of colon cancer cells. ZBP-89 cooperates with histone acetyltransferase coactivator p300 in the regulation of p21, a cyclin-dependent kinase inhibitor whose associated gene is a target gene of p53. ZBP-89 also regulates cell proliferation, in part, through its ability to directly bind the p53 protein and retard its nuclear export. Elevated levels of ZBP-89 induce growth arrest and apoptosis in human gastrointestinal cells.Synonyms: Transcription factor ZBP-89, ZBP89, Zinc finger DNA-binding protein 89, Zinc finger protein 148

Molekulargewicht: approx. 89 kDa

Gen-ID: 7707

NCBI Accession: NP_068799

UniProt: Q9UQR1