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Rhodopsin Antikörper

RHO Reaktivität: Human, Rind (Kuh), Ratte, Maus, Schwein WB, IHC, ICC, IF Wirt: Maus Monoclonal B630 unconjugated
Produktnummer ABIN4956273
  • Target Alle Rhodopsin (RHO) Antikörper anzeigen
    Rhodopsin (RHO)
    Reaktivität
    • 39
    • 17
    • 16
    • 15
    • 10
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Rind (Kuh), Ratte, Maus, Schwein
    Wirt
    • 39
    • 26
    • 2
    • 1
    • 1
    Maus
    Klonalität
    • 35
    • 33
    • 1
    Monoklonal
    Konjugat
    • 28
    • 6
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Dieser Rhodopsin Antikörper ist unkonjugiert
    Applikation
    • 52
    • 42
    • 35
    • 25
    • 14
    • 12
    • 10
    • 8
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
    Aufreinigung
    affinity purified antibody
    Klon
    B630
    Isotyp
    IgG1
    Top Product
    Discover our top product RHO Primärantikörper
  • Applikationshinweise
    Try at dilutions of ~1:1,000 for immunofluorescence. For western blots try at 1:5,000. A suitable control tissue is retinal extracts. Rhodopsin run at 35 kDa on SDS-PAGE gels.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1 mg/mL
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    Store at 4°C short term or -20°C long term.
  • Target
    Rhodopsin (RHO)
    Andere Bezeichnung
    Rhodopsin (RHO Produkte)
    Synonyme
    CSNBAD1 antikoerper, OPN2 antikoerper, RP4 antikoerper, RH1 antikoerper, Rh antikoerper, fi06d11 antikoerper, wu:fi06d11 antikoerper, zfo2 antikoerper, zfrho antikoerper, Noerg1 antikoerper, Opn2 antikoerper, Ops antikoerper, opn2 antikoerper, rh1 antikoerper, rp4 antikoerper, xrho antikoerper, PIGRHO1 antikoerper, ops antikoerper, RDP1 antikoerper, RHO antikoerper, rhodopsin antikoerper, rhodopsin L homeolog antikoerper, RHO antikoerper, Rho antikoerper, rho antikoerper, rho.L antikoerper
    Substanzklasse
    Chemical
    Hintergrund
    Rhodopsin is the protein in the mammalian retina responsible for the light sensitivity of rod cells, which are responsible for vision in low light levels. Somewhat surprisingly, the rhodopsin protein turned out to be a typical member of the seven transmembrane G protein-coupled receptor (GPCR) superfamily. Whereas other GPCRs initiate signaling on binding a specific ligand, rhodopsin exists with a ligand already bound, specifically the vitamin A related substance retinal. Retinal can exist in two isomeric forms, 11-cis and 11-trans retinal. In the dark rhodopsin is associated with 11-cis retinal, but photons cause the 11-cis form to flip to the 11-trans form, and this causes an alteration in the structure of the rhodopsin making it catalytically active. Activated rhodopsin in turn activates the GTP binding protein transducin by favoring the loss of GDP and the addition of GTP. Transducin is a typical member of the family of heterotrimeric G proteins, and consists of an alpha, and a beta,gamma, subunit. The alpha, subunit is responsible for the GTP binding and the GTP bound form activates a phosphodiesterase (PDE) enzyme which hydrolyses cyclic GMP. This in turn results in the closure of cyclic nucleotide gated ion channels in the rod cell membrane, which prevent the influx of sodium and calcium ions. This results in an increase in the membrane potential of the rod cell and so reduces the rate of synaptic signaling. So light stimulation results in a reduced rate of photoreceptor synaptic release. This information is transmitted through neurons of the retina to the visual centers of the brain (see review 1, 2). Rhodopsin activity is shut off by phosphorylation under the influence of rhodopsin kinase, the activity of which results in binding of visual arrestin, which prevents rhodopsin from interacting with and activating more transducin molecules. This basic signaling paradigm proved to be a prototype for understanding how other GPCRs function, as proteins similar to transducin, arrestin and rhodopsin kinase are found in these pathways. MCA-B630 was raised against bovine outer segment discs and shows convincing staining for rhodopsin both on western blots and on sections of retina. The protein is concentrated in rod outer segments. The HGNC name for this protein is RHO.
    Pathways
    WNT Signalweg, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction
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