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Antikörper
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anti-Nestin (NES) (C-Term) Antikörper
Nestin (NES) (C-Term) Antikörper
| Antigen |
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| Synonyme |
FLJ21841, Nbla00170, C78523, ESTM46, AA166324, NES, paranemin |
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Epitope
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| Klonalität |
Polyklonal |
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Wirt
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Reaktivität
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Human (86), Maus (37), Ratte (Rattus) (33), Rind (Kuh) (7), Kaninchen (7), Hund (6), Pferd (4), Schwein (4), Hamster (2), Huhn (1), Schimpanse (1), Affe (1), Schaf (1)
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Applikation
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 Alternativen Immunhistochemie (Paraffinschnitte) (IHC (p)), Western Blot (WB)
Western Blot (WB) (70), Immunfluoreszenz (IF) (61), Durchflusszytometrie (FACS) (50), Immunhistochemie (Paraffinschnitte) (IHC (p)) (35), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (24), Immunpräzipitation (IP) (13), ELISA (12), Immunhistochemie (IHC) (11), Enzyme Immunoassay (EIA) (10), Immunzytochemie (ICC) (5), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (4), Immunoelectron Microscopy (IEM) (2)
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| Produktnummer |
ABIN492681 |
| Menge |
0.1 ml (Varianten) |
| Preis |
220,00 € Zzgl. Versandkosten €20,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 7 bis 10 Werktagen |
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Produktmerkmale
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Synonyms: NES, Nbla00170
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Weitere Bezeichnung
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Nestin
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Gen-ID
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10763
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Swiss-Prot
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P48681
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Immunogen
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Synthetic peptide corresponding to C-terminus of Human Nestin. Remarks: Epitope: C-terminus
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Kreuzreaktivität
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Human
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Isotyp
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IgG (Passende Sekundärantikörper)
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Beschreibung
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Nestin is a class VI intermediate filament expressed in the developing central nervoussystem (CNS) in early embryonic neuroepithelial stem cells. This protein has been widelyused as a predominant marker for stem / progenitor cells, glioma cells, and tumorendothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker toevaluate neovascularity of endothelial cells in tumor.
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Spezifität
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Recognizes NestinCellular localisation: CytoplasmicSpecies Reactivity: Tested: Human. Add. Information: Molecular weight: 220 kDa
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Applikationshinweise
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Western Blot: 1/100 for 1 hour at RT. Immunohistochemistry on Paraffin Sections: 1/100 for 10 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer,pH 6.0 for 10 min followed by cooling at RT for 20 min. Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Positive Control: Kidney. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Reinigung
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Purified
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Buffer
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PBS, pH 7.6 with 1% BSA and 0.09% Sodium Azide
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Lagerung
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Store the antibody undiluted at 2–8°C. Shelf life: One year from despatch.
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Alternativen zu Antigen "Nestin (NES)", Typ "Antikörper" finden
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Wirte
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Kaninchen (80), Maus (30), Huhn (3), Ziege (1)
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Reaktivitäten
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Human (86), Maus (37), Ratte (Rattus) (33), Rind (Kuh) (7), Kaninchen (7), Hund (6), Pferd (4), Schwein (4), Hamster (2), Huhn (1), Schimpanse (1), Affe (1), Schaf (1)
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Applikationen
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Western Blot (WB) (70), Immunfluoreszenz (IF) (61), Durchflusszytometrie (FACS) (50), Immunhistochemie (Paraffinschnitte) (IHC (p)) (35), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (24), Immunpräzipitation (IP) (13), ELISA (12), Immunhistochemie (IHC) (11), Enzyme Immunoassay (EIA) (10), Immunzytochemie (ICC) (5), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (4), Immunoelectron Microscopy (IEM) (2)
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Konjugate
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Biotin (4), HRP (4), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2)
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Epitope
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C-Term (3), AA 1484-1500 (1), AA 544-776 (1)
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