Sodium Channel, Voltage-Gated, Type X, alpha (SCN10A) (AA 1724-1956) Antikörper

Antigen: Sodium Channel, Voltage-Gated, Type X, alpha (SCN10A)

Epitope: AA 1724-1956

Klonalität: Polyklonal

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Immunfluoreszenz (IF), Immunpräzipitation (IP), Western Blot (WB)

Produktnummer: ABIN492472

Produktbeschreibung

Produktmerkmale: Synonyms: Sodium channel protein type 10 subunit alpha, Sodium channel protein type X subunitalpha, Voltage-gated sodium channel subunit alpha Nav1.8, Peripheral nerve sodiumchannel 3

Weitere Bezeichnung: SCN10A / PN3

Gen-ID: 29571

Swiss-Prot: Q62968

Immunogen: Fusion protein amino acids 1724-1956 (cytoplamic C-terminus) of Rat SCN10A/NAV1. 8

Kreuzreaktivität: Human, Maus, Ratte (Rattus)

Isotyp: IgG2a  (Passende Sekundärantikörper)

Beschreibung: Ion channels are integral membrane proteins that help establish and control the smallvoltage gradient across the plasma membrane of living cells by allowing the flow of ionsdown their electrochemical gradient (1). They are present in the membranes that surroundall biological cells because their main function is to regulate the flow of ions across thismembrane. Whereas some ion channels permit the passage of ions based on charge,others conduct based on a ionic species, such as sodium or potassium. Furthermore, insome ion channels, the passage is governed by a gate which is controlled by chemical orelectrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ionchannels, ligandgated, other gating systems and finally those that are classifieddifferently, having more exotic characteristics. The first are voltage- gated ion channelswhich open and close in response to membrane potential. These are then separated intosodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gatedchannels, each of which is responsible for a unique role. Ligand-gated ion channels arealso known as ionotropic receptors, and they open in response to specific ligandmolecules binding to the extracellular domain of the receptor protein. The other gatedclassifications include activation and inactivation by second messengers, inwardrectifierpotassium channels, calcium-activated potassium channels, two-pore-domain potassiumchannels, light-gated channels, mechano-sensitive ion channels and cyclicnucleotide-gated channels. Finally, the other classifications are based on less normalcharacteristics such as two-pore channels, and transient receptor potential channels (2). NAV1. 8 is a voltage-gated sodium channel and plays a critical role in the generation andconduction of action potentials and is thus important for electrical signaling by mostexcitable cells. Therapeutically, the association of pain insensitivity with the loss offunction of a certain sodium channel may have implications. Since Nav1. 8 is not present incardiac muscle or neurons in the central nervous system, blockers of Nav1. 8 will not havedirect action on these cells and thus can have less side effects than current painmedications. By performing more studies, there is a possibility to develop a newgeneration of drugs that can reduce the pain intensity in animals (3).

Spezifität: Detects ~220kDa protein. No cross reactivity against other Nav channels. Species: Human, Mouse and Rat. Others not tested.

Anwendungen

Applikationshinweise: Western blot: 1 µg/ml (if results are poor, use the lysate in SDS buffer but without boiling,incubate for 20 mins at 37°C).1 µg/ml was sufficient for detection of Nav1.8in 10 μg COS cell lysate transiently expressingNav1.8 by colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as thesecondary antibody. Immunoprecipitation: 1.0-10 µg/mlImmunofluorescence: 1.0-10 µg/mlImmunocytochemistry: 0.1-1.0 µg/mlImmunohistochemistry: 0.1-1.0 µg/mlOther applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 1.0 mg/ml

Reinigung: Purified

Buffer: PBS, pH 7.4 containing 50% Glycerol as stabilizer and 0.09% Sodium Azideas preservative.

Lagerung: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Beschränkungen: Nur für Forschungszwecke einsetzbar