STX1A Antikörper

Produktnummer ABIN492371

Produktdetails anti-STX1A Antikörper

Target: STX1A (Syntaxin 1A (Brain) (STX1A))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Kaninchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser STX1A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Spezifität: This antibody reacts with 35 kDa membrane protein Syntaxin-1 expressed predominantly in neuronal tissues on Western blotting, Immunoprecipitation and Immunohistochemistry.

Kreuzreaktivität (Details): Species reactivity (expected):Bovine and Rat.
Species reactivity (tested):Human, Mouse and Rat.

Produktmerkmale: Synonyms: Syntaxin-1A, STX1, Neuron-specific antigen HPC-1

Aufreinigung: Protein-A Sepharose Chromatography of hybridoma supernatant.

Immunogen: Synaptosomal plasma membrane fraction from adult Rat hippocampus. Remarks: This hybridoma was established by fusion of Mouse myeloma cell NSI/Ag4-1 withBalb/c mouse splenocyte.

Klon: HPC-1

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western blotting: 0.1 μg/mL for chemiluminescence detection system. Immunoprecipitation: 2 μg/200 μL of cell extract. Immunohistochemistry on Paraffin Embedded Sections: 5 μg/mL (Heat treatmentnecessary). Microwave oven: 2 times for 10 minutes each in 10 mM citrate buffer ( pH 6.5). Detailed procedure is provided in Protocols.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: SDS-PAGE & Western Blotting1) Wash the cells 3 times with PBS and suspend with 10 volume of cold Lysis buffer (50 mMTris-HCl, pH 7. 2, 250 mM NaCl, 0. 1% NP-40, 2 mM EDTA, 10% glycerol) containingappropriate protease inhibitors. Incubate it at 4°C with rotating for 30 minutes, thensonicate briefly (up to 10 seconds). 2) Centrifuge the tube at 12,000 x g for 10 minutes at 4°C and transfer the supernatant toanother tube. Measure the protein concentration of the supernatant and add the cold lysisbuffer to make 8 mg/ml solution. 3. Mix the sample with equal volume of Laemmli’s sample buffer. 4) Boil the samples for 2 minutes and centrifuge. Load 10 μL of the sample per lane in a 1mm thickSDS-polyacrylamide gel for electrophoresis. 5) Blot the protein to a polyvinylidene difluoride (PVDF) membrane at 1 mA/cm2for 1 hour ina semi-dry transfer system (Transfer Buffer: 25 mM Tris, 190 mM glycine, 20% MeOH). Seethe manufacture's manual for precise transfer procedure. 6) To reduce nonspecific binding, soak the membrane in 10% skimmed milk (in PBS, pH7. 2)for 1 hour at RT or overnight at 4°C. 7) Incubate the membrane with primary antibody diluted with PBS, pH 7. 2 containing 1%skimmed milk as suggest in the APPLICATIONS for 1 hour at room temperature. (Theconcentration of antibody will depend on condition. )8) Wash the membrane with PBS-T [0. 05% Tween-20 in PBS] (5 mimnutes x 3 times). 9) Incubate the membrane with the 1: 10,000 HRP-conjugated anti-mouse IgG diluted with1% skimmed milk (in PBS, pH 7. 2) for 1 hour at RT. 10) Wash the membrane with PBS-T (5 minutes x 6 times). 11) Wipe excess buffer on the membrane, then incubate it with appropriatechemiluminescence reagent for 1 minute. Remove extra reagent from the membrane bydabbing with paper towel, and seal it in plastic wrap. 12) Expose to an X-ray film in a dark room for 5 minutes. Develop the film as usual. Thecondition for exposure and development may vary. Positive Controls: Mouse brain, PC12Immunoprecipitation1) Wash the cells 3 times with PBS and suspend with 10 volume of cold Lysis buffer (50 mMTris-HCl pH 7. 2, 250 mM NaCl, 0. 1% NP-40, 2 mM EDTA, 10% glycerol) containingappropriate protease inhibitors. Incubate it at 4°C with rotating for 30 minutes, thensonicate briefly (up to 10 seconds). 2) Centrifuge the tube at 12,000 x g for 10 minutes at 4°C and transfer the supernatant toanother tube. 3) Add primary antibody as suggest in the APPLICATIONS into 200 µL of the supernatant. Mix well and incubate with gentle agitation for 30-120 minutes at 4°C. Add 20 µl of 50%protein A agarose beads resuspended in the cold lysis buffer. Mix well and incubate withgentle agitation for 60 minutes at 4°C.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 containing 50 % Glycerol without preservatives.

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu STX1A

Target: STX1A (Syntaxin 1A (Brain) (STX1A))

Andere Bezeichnung: Syntaxin 1A / STX1A (STX1A Produkte)

Synonyme: HPC-1 antikoerper, P35-1 antikoerper, STX1 antikoerper, SYN1A antikoerper, STX1A antikoerper, hpc-1 antikoerper, p35-1 antikoerper, stx1 antikoerper, sx1a antikoerper, syntaxin1a antikoerper, CG10716 antikoerper, CG18615 antikoerper, CG31136 antikoerper, CG5448 antikoerper, CT30033 antikoerper, Dm Syx1 antikoerper, DmSyx1A antikoerper, Dmel\\CG31136 antikoerper, SYX1A antikoerper, Syt1A antikoerper, Syx antikoerper, Syx-1A antikoerper, Syx1 antikoerper, Syx1a antikoerper, SyxA antikoerper, anon-EST:Gibbs4 antikoerper, anon-WO02059370.54 antikoerper, dSyx1 antikoerper, l(3)06737 antikoerper, synt antikoerper, syt-1A antikoerper, syx antikoerper, syx-1 antikoerper, syx-1A antikoerper, syx1 antikoerper, syx1A antikoerper, syntaxin 1A antikoerper, syntaxin 1A (brain) antikoerper, Syntaxin 1A antikoerper, syntaxin-1A antikoerper, STX1A antikoerper, Stx1a antikoerper, stx1a antikoerper, Syx1A antikoerper, EDI_005240 antikoerper, Tsp_07354 antikoerper

Hintergrund: Syntaxin 1, also known as HPC-1, is a 35 kDa integral membrane protein that is abundantly expressed in neurons and neuroendocrine cells. Syntaxin is essential for synaptic vesicle fusion and it interacts with several other proteins important for synaptic function, including its partners in the fusion complex (Synaptobrevin/VAMP, SNAP-25, α-SNAP, synaptotagmin, Munc-18/n-Sec1, and Ca2+-channels). Syntaxin is concentrated in synapses and synaptic vesicles where it is a component of the SNARE complex.Synonyms: Neuron-specific antigen HPC-1, STX1, Syntaxin-1A

Gen-ID: 6804

UniProt: Q16623

Pathways: Peptide Hormone Metabolism, Synaptic Membrane, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport