His Tag Antikörper

Details zu Produkt Nr. ABIN4900772, Anbieter: Anmelden zum Anzeigen
Antigen
  • AKR1B11
  • AKR1B12
  • ALDRLn
  • ARL-1
  • ARL1
  • HIS
  • HSI
  • aldo-keto reductase family 1, member B10 (aldose reductase)
  • AKR1B10
Reaktivität
Tag
616
24
22
14
3
2
Wirt
Maus
434
184
27
23
4
4
3
1
Klonalität (Klon)
Monoklonal ()
Konjugat
Unkonjugiert
59
45
36
15
14
12
11
10
10
10
10
9
8
8
8
8
7
7
6
5
4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
Applikation
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Simple Western (SimWes), Western Blotting (WB)
554
386
167
107
98
77
69
26
13
11
9
7
7
6
6
5
5
4
4
3
3
2
1
1
1
1
1
1
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen His-tagged peptide
Klon AD1-1-10
Isotyp IgG1
Spezifität Detects proteins containing accessible consecutive histidine regions. The antibody detects His tags localized at the amino- or carboxyl-terminus.
Reinigung Protein A or G purified from hybridoma culture supernatant
Sterilität 0.2 μm filtered
Endotoxin-Niveau <0.20 EU per 1 μg of the antibody by the LAL method.
Substanzklasse Tag
Hintergrund Alternative Names: 6 His epitope tag, 6X His, 6X-His, H, HHHHHH epitope tag, HHHHHH tag, HIS, His tag, polyHistidine, Poly-histidine
Forschungsgebiet Tags/Labels
Applikationshinweise Western Blot 0.2 μg/mL
Simple Western 10 μg/mL
Flow Cytometry 0.25 μg/10^6 cells
Immunoaffinity Purification
CyTOF-ready
Beschränkungen Nur für Forschungszwecke einsetzbar
Rekonstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Konservierungsmittel Without preservative
Lagerung 4 °C,-20 °C,-80 °C
Informationen zur Lagerung Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Haltbarkeit 12 months
Bilder des Herstellers
Flow Cytometry (FACS) image for anti-His Tag antibody (ABIN4900772) Detection of His Tag in HEK293 Human Cell Line Transfected with His-tagged Proteins b...
Simple Western (SimWes) image for anti-His Tag antibody (ABIN4900772) Detection of His Tag by Simple WesternTM. Simple Western lane view shows lysates of C...
Western Blotting (WB) image for anti-His Tag antibody (ABIN4900772) Detection of His Tag by Western Blot. Western blot shows lysates of HEK293 human embr...
Flow Cytometry (FACS) image for anti-His Tag antibody (ABIN4900772) Detection of His Tag in HEK293 Human Cell Line Transfected with His-tagged Proteins b...
Simple Western (SimWes) image for anti-His Tag antibody (ABIN4900772) Detection of His Tag by Simple WesternTM. Simple Western lane view shows lysates of C...
Western Blotting (WB) image for anti-His Tag antibody (ABIN4900772) Detection of His Tag by Western Blot. Western blot shows lysates of HEK293 human embr...
Produkt verwendet in: Griffiths, Dolezal, Cao, Nilsson, See, Pfleger, Roche, Gorry, Pow, Viduka, Lim, Lu, Chang, Murray-Rust, Kvansakul, Perugini, Dogovski, Doerflinger, Zhang, Parisi, Casey, Nuttall, Foley: "i-bodies, Human Single Domain Antibodies That Antagonize Chemokine Receptor CXCR4." in: The Journal of biological chemistry, Vol. 291, Issue 24, pp. 12641-57, 2016 (PubMed). Von den Autoren verwendete Methode: Biochemical Assay (BCA) (Probematerial (Species): Human).

Chang, Li, Kaplan: "Endotoxemia contributes to CD27+ memory B-cell apoptosis via enhanced sensitivity to Fas ligation in patients with Cirrhosis." in: Scientific reports, Vol. 6, pp. 36862, 2016 (PubMed). (Probematerial (Species): Human).

Feng, Tran, Bale, Kumar, Guenaga, Wilson, de Val, Arendt, DeStefano, Ward, Wyatt: "Thermostability of Well-Ordered HIV Spikes Correlates with the Elicitation of Autologous Tier 2 Neutralizing Antibodies." in: PLoS pathogens, Vol. 12, Issue 8, pp. e1005767, 2016 (PubMed). Von den Autoren verwendete Methode: ELISA (Capture) (ELISA (Capture)) (Probematerial (Species): Guinea Pig).

Moores, Chiu, Bushey, Chevalier, Luistro, Dorn, Brezski, Haytko, Kelly, Wu, Martin, Neijssen, Parren, Schuurman, Attar, Laquerre, Lorenzi, Anderson: "A Novel Bispecific Antibody Targeting EGFR and cMet Is Effective against EGFR Inhibitor-Resistant Lung Tumors." in: Cancer research, Vol. 76, Issue 13, pp. 3942-53, 2016 (PubMed). Von den Autoren verwendete Methode: Functional Studies (Func) (Probematerial (Species): Human).

Reches, Nachmani, Berhani, Duev-Cohen, Shreibman, Ophir, Seliger, Mandelboim: "HNRNPR Regulates the Expression of Classical and Nonclassical MHC Class I Proteins." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 196, Issue 12, pp. 4967-76, 2016 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Human).

Konitsiotis, Jovanović, Ciepla, Spitaler, Lanyon-Hogg, Tate, Magee: "Topological analysis of Hedgehog acyltransferase, a multipalmitoylated transmembrane protein." in: The Journal of biological chemistry, Vol. 290, Issue 6, pp. 3293-307, 2015 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Human).

La Porte, Eigenbrot, Ultsch, Ho, Foletti, Forgie, Lindquist, Shelton, Pons: "Generation of a high-fidelity antibody against nerve growth factor using library scanning mutagenesis and validation with structures of the initial and optimized Fab-antigen complexes." in: mAbs, Vol. 6, Issue 4, pp. 1059-68, 2014 (PubMed).

Kyathanahalli, Marks, Nye, Lao, Albrecht, Aberdeen, Nathanielsz, Jeyasuria, Condon: "Cross-species withdrawal of MCL1 facilitates postpartum uterine involution in both the mouse and baboon." in: Endocrinology, Vol. 154, Issue 12, pp. 4873-84, 2013 (PubMed). (Probematerial (Species): Human).

Matsui, Higashimoto, Taira, Yamagishi: "Pigment epithelium-derived factor (PEDF) binds to caveolin-1 and inhibits the pro-inflammatory effects of caveolin-1 in endothelial cells." in: Biochemical and biophysical research communications, Vol. 441, Issue 2, pp. 405-10, 2013 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Human).

Ricciardi, Malpeli, Bifari, Bassi, Pacelli, Nwabo Kamdje, Chilosi, Krampera: "Comparison of epithelial differentiation and immune regulatory properties of mesenchymal stromal cells derived from human lung and bone marrow." in: PLoS ONE, Vol. 7, Issue 5, pp. e35639, 2012 (PubMed). (Probematerial (Species): Human).

Simhadri, Hansen, Simhadri, Reiners, Bessler, Engert, von Strandmann: "A novel role for reciprocal CD30-CD30L signaling in the cross-talk between natural killer and dendritic cells." in: Biological chemistry, Vol. 393, Issue 1-2, pp. 101-6, 2012 (PubMed). (Probematerial (Species): Others).

Stephenson-Famy, Marks, Suresh, Caritis, Simhan, Jeyasuria, Condon: "Antiapoptotic signaling via MCL1 confers resistance to caspase-3-mediated apoptotic cell death in the pregnant human uterine myocyte." in: Molecular endocrinology (Baltimore, Md.), Vol. 26, Issue 2, pp. 320-30, 2012 (PubMed). (Probematerial (Species): Others).

Chao, Joyce-Shaikh, Grein, Moshrefi, Raoufi, Laface, McClanahan, Bourne, Pierce, Gorman, Pflanz: "C17 prevents inflammatory arthritis and associated joint destruction in mice." in: PLoS ONE, Vol. 6, Issue 7, pp. e22256, 2011 (PubMed). Von den Autoren verwendete Methode: ELISA (ELISA) (Probematerial (Species): Mouse (Murine)).

Wu, Ethen, Hickey, Jiang: "Active 1918 pandemic flu viral neuraminidase has distinct N-glycan profile and is resistant to trypsin digestion." in: Biochemical and biophysical research communications, Vol. 379, Issue 3, pp. 749-53, 2009 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Virus).

Sengle, Ono, Lyons, Bächinger, Sakai: "A new model for growth factor activation: type II receptors compete with the prodomain for BMP-7." in: Journal of molecular biology, Vol. 381, Issue 4, pp. 1025-39, 2008 (PubMed). Von den Autoren verwendete Methode: ELISA (ELISA) (Probematerial (Species): Human).

Sengle, Charbonneau, Ono, Sasaki, Alvarez, Keene, Bächinger, Sakai: "Targeting of bone morphogenetic protein growth factor complexes to fibrillin." in: The Journal of biological chemistry, Vol. 283, Issue 20, pp. 13874-88, 2008 (PubMed). (Probematerial (Species): Others).

Kovacić, Lukić, Grcević, Katavić, Croucher, Marusić: "The Fas/Fas ligand system inhibits differentiation of murine osteoblasts but has a limited role in osteoblast and osteoclast apoptosis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 6, pp. 3379-89, 2007 (PubMed). (Probematerial (Species): Others).

Fransson, Tornberg, Borrebaeck, Carlsson, Frendéus: "Rapid induction of apoptosis in B-cell lymphoma by functionally isolated human antibodies." in: International journal of cancer, Vol. 119, Issue 2, pp. 349-58, 2006 (PubMed). Von den Autoren verwendete Methode: Flow Cytometry (FACS) (Probematerial (Species): Human).

Cortesio, Jiang: "Mannan-binding lectin-associated serine protease 3 cleaves synthetic peptides and insulin-like growth factor-binding protein 5." in: Archives of biochemistry and biophysics, Vol. 449, Issue 1-2, pp. 164-70, 2006 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Others).

Lee, Haskell, Charo, Boettiger: "Receptor-ligand binding in the cell-substrate contact zone: a quantitative analysis using CX3CR1 and CXCR1 chemokine receptors." in: Biochemistry, Vol. 43, Issue 22, pp. 7179-86, 2004 (PubMed). Von den Autoren verwendete Methode: Binding Studies (Bind) (Probematerial (Species): Mouse (Murine)).

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