CA3 Antikörper (AA 14-235)

Produktnummer ABIN4886494

Produktdetails anti-CA3 Antikörper

Target: CA3 (Carbonic Anhydrase III (CA3))

Bindungsspezifität: AA 14-235

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CA3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Carbonic anhydrase 3(CA3) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Carbonic anhydrase 3(CA3) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: carbonic anhydrase III, muscle specific
Protein Name: Carbonic anhydrase 3

Aufreinigung: Immunogen affinity purified.

Immunogen: E. coli-derived human CA3 recombinant protein (Position: D14-E235). Human CA3 shares 92.3% and 91.4% amino acid (aa) sequence identity with mouse and rat CA3, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse, Rat, Predicted Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Details zu CA3

Target: CA3 (Carbonic Anhydrase III (CA3))

Andere Bezeichnung: CA3 (CA3 Produkte)

Synonyme: CAIII antikoerper, Car3 antikoerper, BB219044 antikoerper, Ca3 antikoerper, Car-3 antikoerper, carbonic anhydrase 3 antikoerper, CA3 antikoerper, CAH3 antikoerper, Car3 antikoerper, Ca3 antikoerper

Hintergrund: Carbonic anhydrase III (CA3) is an enzyme that in humans is encoded by the CA3 gene. CA3 is a member of a multigene family (at least six separate genes are known) that encode carbonic anhydrase isozymes. The gene spans 10.3 kb and contains seven exons and six introns. Using a cDNA clone of the CA3 gene in the study of human-rodent hybrids, the gene was mapped to chromosome 8 which carries a cluster of CA genes. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal.

Synonyms: CA3 | CAH3 | CAIII | CA-III | Carbonic Anhydrase III | P07451

Gen-ID: 761

UniProt: P07451