Structural maintenance of chromosomes protein 1A Antikörper

Antigen: Structural maintenance of chromosomes protein 1A

Klonalität: Monoklonal (8E6-A4-F3)

Wirt: Maus

Reaktivität: Human

Applikation: ELISA, Western Blot (WB), Immunhistochemie (IHC)

Produktnummer: ABIN488213

Produktbeschreibung

Gen-ID: SMC1A

Swiss-Prot: Q14683

Immunogen: Recombinant fragment, corresponding to amino acids 2aa-351aa of human SMC1A(N-terminus).

Format: Purified

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: 8E6-A4-F3

Beschreibung: Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1L2 or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. Caution hazardous ingredient: Sodium azide, avoid exposure to skin and eyes, ingestion, and contact with heat, acids, and metals or metal ions. Wash exposed skin with soap and water. Flush eyes with water. Do not discard into plumbing.

Spezifität: Mouse monoclonal to SMC1A

Anwendungen

Applikationshinweise: ELISA: 1/5000 – 1/20000. WB: 1/100-1/2000

Konzentration: 1. 000mg/ml

Reinheit: > 90%

Reinigung: Protein G-Sepharose affinity purified

Buffer: Preservative: 0. 02% Sodium Azide and 50% glycerol, 1% BSA, PBS, PH7. 4.

Lagerung: Store at -20°C. Avoid repeated freeze / thaw cycles. Ship at 4°-10°

Forschungsgebiet: Chromatin und nukleäre Signaltransduktion, Zellzyklus, Chromatin, DNS/RNS, Zellstruktur

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

SMC1A antibody at 1/200 dilution, Neuclear extracts of HeLa cell 40ug/Lane, Predicted band size: 150kDa, Observed band size: 150kDa

IHC of paraffin-embedded human colon using anti-SMC1A (N-terminus) diluted 1/500-1/1000.