Chemokine (C-C Motif) Receptor 4 (CCR4) Antikörper

Antigen: Chemokine (C-C Motif) Receptor 4 (CCR4)

Synonyme: CKR4, K5-5, CD194, CMKBR4, ChemR13, CC-CKR-4, MGC88293, HGCN:14099

Klonalität: Monoklonal (KH-4F5)

Wirt: Maus

Reaktivität: Human

Applikation: Enzyme Immunoassay (EIA), Durchflusszytometrie (FACS), Immunfluoreszenz (IF)

Produktnummer: ABIN488138

Produktbeschreibung

Produktmerkmale: Synonyms: C-C chemokine receptor type 4, C-C CKR-4, CC-CKR-4, CCR-4, CMKBR4, K5-5

Weitere Bezeichnung: CD194 / CCR4

Gen-ID: 1233

Swiss-Prot: P51679

Immunogen: Immunisation with Human CCR4 peptide. Remarks: Selection: Based on recognition of the complete native protein expressed ontransfected mammalian cells

Kreuzreaktivität: Human

Isotyp: IgG2a  (Passende Sekundärantikörper)

Klon: KH-4F5

Beschreibung: CCR4 (CC chemokine receptor 4) belongs to the rhodopsin family of G-protein-coupledreceptors. Chemokine receptors are membrane-bound molecules composed of7-transmembrane domains and are coupled to G-proteins (1,2). CCR4 binds thechemokines CCL17 (TARC) and CCL22 (MDC) and is highly expressed in most single-positiveCD4(+) thymocytes especially in TH2- and regulatory T-cells (3,4). It plays a central role in Tcell migration to the thymus, T cell maturation and education. CCR4 is often up-regulatedin inflammation and cause conformational changes that trigger intracellular signalingpathways (2,5).

Spezifität: Recognizes CCR4. Species Reactivity: Tested: Human. Add. Information: SDS-PAGE analysis: The antibody was purified by protein G affinity chromatography fromcell culture supernatants and verified by SDS-Page (Figure. 3).

Anwendungen

Applikationshinweise: cELISA: 1/200-1/400. Flow Cytometry: 1.2 µg/10e6 cells. Immunofluorescence: 1 µg/10e6 cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 2.0 mg/ml

Reinigung: Purified

Buffer: Phosphate buffered saline, pH 7.2

Lagerung: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Forschungsgebiet: Chemokine, Rezeptoren

Beschränkungen: Nur für Forschungszwecke einsetzbar