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CDKN2D Antikörper

CDKN2D Reaktivität: Human WB, IP, IHC (p) Wirt: Maus Monoclonal DCS-100 unconjugated
Produktnummer ABIN487338
  • Target Alle CDKN2D Antikörper anzeigen
    CDKN2D (Cyclin-Dependent Kinase Inhibitor 2D (p19, Inhibits CDK4) (CDKN2D))
    Reaktivität
    • 43
    • 12
    • 3
    Human
    Wirt
    • 34
    • 10
    Maus
    Klonalität
    • 35
    • 9
    Monoklonal
    Konjugat
    • 23
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser CDKN2D Antikörper ist unkonjugiert
    Applikation
    • 38
    • 16
    • 15
    • 13
    • 13
    • 9
    • 8
    • 7
    • 5
    • 4
    • 3
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Spezifität
    This antibody reacts with Human p19INK4d
    Kreuzreaktivität (Details)
    Species reactivity (tested):Human.
    Produktmerkmale
    Synonyms: p19-INK4d, p19, INK4D, Cyclin-dependent kinase 4 inhibitor D
    Aufreinigung
    Protein-A Sepharose Chromatography.
    Immunogen
    Bacterially produced GST-tagged fusion proteins of full-length p19INK4d. Remarks: Hybridoma was established by fusion of Mouse myeloma cell NS-2 with Balb/cmouse splenocyte.
    Klon
    DCS-100
    Isotyp
    IgG1
    Top Product
    Discover our top product CDKN2D Primärantikörper
  • Applikationshinweise
    Western Blot: 1 μg/mLPositive Control: Jurkat cells. Immunoprecipitation: 3 μg/200-300 μL of cell extract. Positive Control: Jurkat cells. Immunohistochemistry: 1-5 μg/mLHeat treatment is necessary for Paraffin Embedded Sections. Microwave oven: 2 times for 10 minutes each in citrate buffer ( pH 6.5). Positive Control: Tonsil Tissue. Detailed procedure is provided in Protocols.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Protokoll
    SDS-PAGE & Western Blotting1) Wash the cells 3 times with PBS and suspend with 10 volume of cold Lysis buffer (50 mMTris-HCl, pH 7. 2, 250 mM NaCl, 0. 1% NP-40, 2 mM EDTA, 10% glycerol) containingappropriate protease inhibitors. Incubate it at 4°C with rotating for 30 minutes, thensonicate briefly (up to 10 seconds). 2) Centrifuge the tube at 12,000 x g for 10 minutes at 4°C and transfer the supernatant toanother tube. Measure the protein concentration of the supernatant and add the Lysisbuffer to make 8 mg/mL solution. 3) Mix the sample with equal volume of Laemmli’s sample buffer. 4) Boil the samples for 2 minutes and centrifuge. Load 10 μL of the sample per lane in a 1mm thick SDS-polyacrylamide gel for electrophoresis. 5) Blot the protein to a polyvinylidene difluoride (PVDF) membrane at 1 mA/cm2 for 1 hourin a semi-dry transfer system. (Transfer Buffer: 25 mM Tris, 190 mM glycine, 20% MeOH). See the manufacture's manual for the transfer procedure. 6) To reduce nonspecific binding, soak the membrane in 10% skimmed milk (in PBS, pH7. 2) for 1 hour at room temperature, or overnight at 4°C. 7) Incubate the membrane with the anti-p19INK4d (DCS-100) monoclonal antibody (1μg/mL) diluted with 1% skimmed milk (in PBS, pH 7. 2) for 1 hour at room temperature. 8) Wash the membrane with PBS (5 minutes x 6 times). 9) Incubate the membrane with the 1: 10000 HRP-conjugated anti-mouse IgG diluted with1% skimmed milk (in PBS, pH 7. 2) for 1 hour at room temperature. 10) Wash the membrane with PBS (5 minutes x 6 times). 11) Wipe excess buffer from the membrane, then incubate it with appropriatechemiluminescence reagents for 1 minute. Remove extra reagent from the membrane bydabbing with a paper towel, and seal it in plastic wrap. 12) Expose to an X-ray film in a dark room for 5 minutes. Develop the film as usual. Theconditions for exposure and development may vary. Positive Control for Western blotting: Jurkat Cells. Immunoprecipitation1) Wash the cells 3 times with PBS and suspend with 10 volume of cold Lysis buffer (50 mMTris-HCl, pH 7. 2, 250 mM NaCl, 0. 1% NP-40, 2 mM EDTA, 10% glycerol) containingappropriate protease inhibitors. Incubate it at 4°C with rotating for 30 minutes, thensonicate briefly (up to 10 seconds). 2) Centrifuge the tube at 12,000 x g for 10 minutes at 4°C and transfer the supernatant toanother tube. 3) Add 3 μg of the anti-p19INK4d (DCS-100) monoclonal antibody into 250 μL of thesupernatant. Mix well and incubate with gentle agitation for 30-120 minutes at 4°C. Add 20μL of 50% Protein A-agarose beads resuspended in the Lysis buffer. Mix well and incubatewith gentle agitation for 60 minutes at 4°C. 4) Wash the beads 3-5 times with ice-cold Lysis buffer (centrifuge the tube at 2,500 x g for10 seconds). 5) Resuspend the beads in 20 μL of Laemmli’s sample buffer, boil for 3-5 minutes, andcentrifuge for 5 minutes. Use 10 μL/lane for the SDS-PAGE analysis. (See SDS-PAGE & Western blotting. )Positive Controls for immunoprecipitation: Jurkat cells.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1.0 mg/mL
    Buffer
    PBS, pH 7.2 containing 50 % Glycerol without preservatives.
    Konservierungsmittel
    Without preservative
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Store the antibody undiluted at -20 °C.
    Shelf life: one year from despatch.
    Haltbarkeit
    12 months
  • Target
    CDKN2D (Cyclin-Dependent Kinase Inhibitor 2D (p19, Inhibits CDK4) (CDKN2D))
    Andere Bezeichnung
    CDKN2D / p19INK4d (CDKN2D Produkte)
    Synonyme
    ink4d antikoerper, p19 antikoerper, p19-ink4d antikoerper, CDKN2D antikoerper, ank2 antikoerper, INK4D antikoerper, p19-INK4D antikoerper, INK4d antikoerper, p19INK4d antikoerper, cyclin-dependent kinase inhibitor 2D S homeolog antikoerper, cyclin dependent kinase inhibitor 2D antikoerper, cyclin-dependent kinase inhibitor 2D antikoerper, Cyclin-dependent kinase 4 inhibitor D antikoerper, cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4) antikoerper, similar to cyclin-dependent kinase inhibitor 2D antikoerper, cdkn2d.S antikoerper, CDKN2D antikoerper, cdkn2d antikoerper, cdn2d antikoerper, Cdkn2d antikoerper
    Hintergrund
    The INK4 family of proteins consists of four members that block progression from the G(1)-to-S phase of the cell cycle by inhibiting the activity of Cdk4 and Cdk6. p19INK4d is a 165 amino acid protein with strong structural and functional similarity to p16INK4a, a known tumor suppressor. Mutations in p19INK4d have also been associated with human osteosarcomas.Synonyms: Cyclin-dependent kinase 4 inhibitor D, INK4D, p19, p19-INK4d
    Gen-ID
    1032
    UniProt
    P55273
    Pathways
    Zellzyklus, Sensory Perception of Sound, Mitotic G1-G1/S Phases, Negative Regulation of intrinsic apoptotic Signaling
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