XCL1 Antikörper

Produktnummer ABIN465561

Der Kaninchen Polyklonal anti-XCL1 Antikörper (ABIN465561) detektiert spezifisch XCL1 in WB, ELISA und Neut. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für XCL1 Antikörper (ABIN465561)

Target: XCL1 (Chemokine (C Motif) Ligand 1 (XCL1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser XCL1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Neutralization (Neut)

Produktdetails anti-XCL1 Antikörper

Spezifität: Human Lymphotactin

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human Lymphotactin.

Type of Immunogen: Cell extract

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 2 μg/mL), Neut (1.7 - 3 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human normal kidney. The recommended concentration is 0.5 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hLymphotactin (60 ng/mL), a concentration of 1.7-3 μg/mL of this antibody is required. ELISA: To detect hLymphotactin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Lymphotactin (LS-C104636) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hLymphotactin. Western Blot: To detect hLymphotactin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLymphotactin is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile water

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu XCL1

Target: XCL1 (Chemokine (C Motif) Ligand 1 (XCL1))

Andere Bezeichnung: XCL1 / Lymphotactin

Hintergrund: Name/Gene ID: XCL1
Subfamily: Intercrine gamma
Family: Intercrine

Synonyms: XCL1, ATAC, C motif chemokine 1, Chemokine (C motif) ligand 1, Cytokine SCM-1, Cytokine scm, LPTN, SCM-1, SCM-1-alpha, SCM-1a, SCM1, SCYC1, Scyc2, Single cysteine motif 1a, Lymphotaxin, Xc chemokine ligand, LTN, Lymphotactin, SCM1A, Small-inducible cytokine C1, XC chemokine ligand 1

Gen-ID: 6375

UniProt: P47992

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation