eIF4EBP2 Antikörper (AA 99-120)

Produktnummer ABIN462435

Produktdetails anti-eIF4EBP2 Antikörper

Target: eIF4EBP2 (EIF4EBP2) (Eukaryotic Translation Initiation Factor 4E Binding Protein 2 (EIF4EBP2))

Bindungsspezifität: AA 99-120

Reaktivität: Human, Maus, Rind (Kuh), Hund, Schwein, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser eIF4EBP2 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Detects 17 and 20 kD proteins, corresponding to the apparent molecular mass of PHAS-II and its phosphorylated state on SDS-PAGE immunoblots.

Aufreinigung: Immunoaffinity purified

Immunogen: Human PHAS-II synthetic peptide (residues 99-120) was synthesized and the peptide coupled to KLH. This sequence is 96% homologous to mouse PHAS-11 over these residues.

Type of Immunogen: Synthetic peptide - KLH conjugated

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (10 μg/mL), WB (4 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: BBS,  pH 8.4 (25 mM sodium borate, 100 mM boric acid, 75 mM sodium chloride, 5 mM EDTA)

Handhabung: avoid freeze thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.

Details zu eIF4EBP2

Target: eIF4EBP2 (EIF4EBP2) (Eukaryotic Translation Initiation Factor 4E Binding Protein 2 (EIF4EBP2))

Andere Bezeichnung: 4E-BP2 / EIF4EBP2 (EIF4EBP2 Produkte)

Synonyme: 4ebp2 antikoerper, MGC75579 antikoerper, wu:fa55e11 antikoerper, wu:fp45e09 antikoerper, MGC78987 antikoerper, EIF4EBP2 antikoerper, eIF-4E antikoerper, Tb06.28P18.1010 antikoerper, 4EBP2 antikoerper, PHASII antikoerper, 2810011I19Rik antikoerper, 4E-BP2 antikoerper, AA792569 antikoerper, BC010348 antikoerper, PHAS-II antikoerper, eukaryotic translation initiation factor 4E binding protein 2 antikoerper, eukaryotic translation initiation factor 4E binding protein 2 L homeolog antikoerper, eukaryotic initiation factor 4E antikoerper, eukaryotic translation initiation factor 4E antikoerper, putative eukaryotic translation initiation factor 4e antikoerper, eif4ebp2 antikoerper, Eif4ebp2 antikoerper, eif4ebp2.L antikoerper, EIF4EBP2 antikoerper, eIF-4E antikoerper, Tb927.6.1870 antikoerper, LMJF_30_0450 antikoerper

Hintergrund: Name/Gene ID: EIF4EBP2

Synonyms: EIF4EBP2, 4E-BP2, 4EBP2, EIF4E-binding protein 2, PHASII

Gen-ID: 1979

UniProt: Q13542