P4HA1 Antikörper (AA 431-443)

Produktnummer ABIN461984

Produktdetails anti-P4HA1 Antikörper

Target: P4HA1 (Prolyl 4-Hydroxylase, alpha Polypeptide I (P4HA1))

Bindungsspezifität: AA 431-443

Reaktivität: Human, Hund, Schwein, Rind (Kuh), Pferd, Kaninchen, Affe, Fledermaus, Huhn

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser P4HA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human P4HA1. This antibody is expected to recognise both reported isoforms (NP_000908.2 and NP_001017962.1).

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Marmoset, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Turkey, Chicken, Platypus, Lizard (100%) Mouse, Rat, Hamster, Opossum, Salmon, Stickleback, Pufferfish, Zebrafish (92%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-DFARKDEPDAFKE from an internal region of human P4HA1 (NP_000908.2, NP_001017962.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Marmoset, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Turkey, Chicken, Platypus, Lizard (100%), Mouse, Rat, Hamster, Opossum, Salmon, Stickleback, Pufferfish, Zebrafish (92%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000), WB (1:32000, 0.1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu P4HA1

Target: P4HA1 (Prolyl 4-Hydroxylase, alpha Polypeptide I (P4HA1))

Andere Bezeichnung: P4HA1 (P4HA1 Produkte)

Synonyme: P4HA antikoerper, AL022634 antikoerper, P4ha antikoerper, PHalphaI antikoerper, prolyl 4-hydroxylase subunit alpha-1 antikoerper, prolyl 4-hydroxylase subunit alpha 1 antikoerper, procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha 1 polypeptide antikoerper, CpipJ_CPIJ000975 antikoerper, CpipJ_CPIJ017131 antikoerper, Tsp_03346 antikoerper, Tsp_03347 antikoerper, Tsp_15957 antikoerper, P4HA1 antikoerper, P4ha1 antikoerper

Hintergrund: Name/Gene ID: P4HA1

Synonyms: P4HA1, 4-PH alpha-1, C-P4Halpha(I), p4HA, p4HA1

Gen-ID: 5033

NCBI Accession: NP_000908, NP_001017962

UniProt: P13674