In den Warenkorb
Bestellen Sie auch über:
 |
+49 (0)241 95 163 153 |
 |
+49 (0)241 95 163 155 |
V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antikörper
| Antigen | V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) |
| Synonyme | NS5, CRAF, Raf-1, c-Raf, craf, c-raf, RAF1, MGC165882, raf1, DKFZp469F0314 |
| Klonalität | Polyklonal |
| Wirt |
 Alternativen Kaninchen |
| Reaktivität |
Alternativen Human, Ratte (Rattus) |
| Applikation |
Alternativen Western Blot (WB), Immunhistochemie (IHC)
|
| Produktnummer | ABIN461615 |
| Menge | 100µg (1 mg/ml) (Varianten) |
| Preis | 572,09 € Zzgl. Versandkosten €20,00 und MWSt |
| Lieferung nach |
|
| Verfügbarkeit | Lieferung in 7 bis 10 Werktagen |
Produktbeschreibung
| Weitere Bezeichnung | c-RAF |
| Format | Liquid |
| Beschreibung |
The Raf family of serine/threonine-specific kinases is comprised of three members (A-Raf, B-Raf, and c-Raf) that play a critical role in regulating cell growth and differentiation, and couple growth factor receptor stimulation to nuclear transcription factors via the Ras/mitogen-activated protein kinase (MAPK) pathway. c-Raf kinase (also known as Raf-1) is a key 74 kDa signal transducer of multiple extracellular stimuli that is regulated by several pathways, and that once activated, phosphorylates MEK which in turn phosphorylates ERK. Activation of Raf kinase activity is induced by several events including phosphorylation at several sites and binding of GTP-bound Ras and/or 14-3-3 proteins. The c-Raf antibody is useful as a positive control and for measuring total c-Raf protein levels to compare signals obtained with the c-Raf PSSAs. Synonyms: EC 2.7.11.1, C-RAF, cRaf, Raf-1, c-Raf, 5894, RAF1, 164760, P04049, CRAF, Raf-1, c-Raf, |
| Spezifität | C-RAF (M265) pAb detects endogenous levels of c-RAF protein. |
| Molekulargewicht | 73 kDa |
Anwendungen
| Applikationshinweise | WB: 1:500-1:1000 IHC: 1:50-1:200 |
| Konzentration | 1 mg/ml |
| Reinheit | > 95% (by SDS-PAGE). |
| Reinigung | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Buffer | 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2. |
| Lagerung | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Beschränkungen | Nur für Forschungszwecke einsetzbar |
Bilder
| anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) antibody anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) antibody (Image 2) |
Alternativen
Alternativen zu Antigen "V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1)", Typ "Antikörper" finden
| Wirte | Kaninchen (135), Maus (15), Ratte (8), Huhn (1) |
| Reaktivitäten | Human (156), Maus (57), Ratte (Rattus) (44), Rind (Kuh) (5), Huhn (3), Hund (3), Xenopus laevis (2), Affe (1) |
| Applikationen | Western Blot (WB) (124), Enzyme Immunoassay (EIA) (55), Immunhistochemie (Paraffinschnitte) (IHC (p)) (45), Immunhistochemie (IHC) (28), Dot Blot (Dot) (19), ELISA (18), Immunfluoreszenz (IF) (7), Immunpräzipitation (IP) (6), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (4), ELISA (Detektionsantikörper) (1), Immunzytochemie (ICC) (1) |
| Epitope | pSer338 (14), N-Term (6), pSer259 (6), pSer642 (4), pSer301 (3), pSer43 (3), S296 (2), S43 (2), S471 (2), T269 (2), Y340 (2), pSer233 (2), pSer296 (2), pSer339 (2), pSer471 (2), pSer494 (2), pSer621 (2), pThr269 (2), pTyr340 (2), pTyr341 (2), S233 (1), S259 (1), S338 (1), S494 (1), S621 (1), Y341 (1), pSer239 (1), pSer338/pTyr340 (1) |
