Forkhead Box O1 (FOXO1) (pSer319) Antikörper

Antigen: Forkhead Box O1 (FOXO1)

Synonyme: FKH1, FKHR, FOXO1A, Afxh, Fkhr1, Foxo1a, AI876417, Fkhr, FOXO1, fkh1, fkhr, foxo1a, xFoxO1, MGC79506

Epitope: pSer319

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Ratte (Rattus)

Applikation: Western Blot (WB), Immunhistochemie (IHC)

Produktnummer: ABIN461508

Produktbeschreibung

Weitere Bezeichnung: FKHR/FOXO1A

Format: Liquid

Beschreibung: The transcription factor forkhead in rhabdomyosarcoma (FKHR), which is inhibited by insulin and IGF-1, enhances transcription. FKHR has been implicated in alveolar rhabdomyosarcoma, a soft tissue tumor wherein a chromosomal translocation [t(2,12)(q35,q14)] occurs between the FKHR and PAX3 genes, resulting in a novel chimeric protein with abnormal levels of expression. FKHR becomes phosphorylated at Ser 319, Ser 256 and Thr 256 by protein kinase B (PKB) in a phosphoinsoditide 3 (PI 3)-kinase/Akt-dependent pathway, resulting in the inactivation and subsequent nuclear exit of FKHR. In addition, FKHR becomes phosphorylated at Ser 329, also resulting in decreased FKHR activity and diminished nuclear FKHR concentration. However, phosphorylation of FKHR at Ser 329 is not mediated by a PI 3-kinase-dependent pathway, but by an alternate mechanism. Dual-specificity tyrosine-phosphorylated and regulated kinase 1A (DYRK1A), which co-localizes to the same region of the nucleus as FKHR, specifically phosphorylates FKHR at Ser 329 in rabbit skeletal muscle.
Synonyms: FOXO1

Spezifität: P-FKHR/FOXO1A (S319) pAb detects endogenous levels of FKHR/FOXO1A protein only when phosphorylated at S319.

Molekulargewicht: 82 kDa

Anwendungen

Applikationshinweise: WB: 1:500-1:1000 IHC: 1:50-1:200

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE).

Reinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Buffer: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Lagerung: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-Forkhead Box O1 (FOXO1) (pSer319) antibody

anti-Forkhead Box O1 (FOXO1) (pSer319) antibody (Image 2)