ZA70 (pTyr493) Antikörper

Antigen: ZA70

Epitope: pTyr493

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Ratte (Rattus)

Applikation: Western Blot (WB), Immunhistochemie (IHC)

Produktnummer: ABIN461441

Produktbeschreibung

Format: Liquid

Beschreibung: The activation of T lymphocytes by antigens is mediated by the T cell receptor (TCR), which is a multisubunit complex assembled from at least six different genes. The TCR subunits include the Ti alpha and beta chains, the CD3 gamma, delta and epsilon chains and a zeta-containing homodimer or heterodimer. The protein tyrosine kinase ZAP-70 binds to the phosphorylated immunoreceptor tyrosine-base activation motifs (ITAMs) of the TCR zeta chain through two Src homology (SH2) domains. This binding results in the phosphorylation of ZAP-70 on multiple tyrosine residues, including Tyr 292 and Tyr 319. ZAP-70 is autophosphorylated on Tyr 292, which is thought to negatively regulate ZAP-70 function in lymphocytes. Alternatively, ZAP-70 is positively regulated by phosphorylation on Tyr 319, which mediates the SH2-dependent interaction between Lck and ZAP-70.

Spezifität: P-ZAP-70 (Y493) pAb detects endogenous levels of ZAP-70 protein only when phosphorylated at Y493.

Molekulargewicht: 70 kDa

Anwendungen

Applikationshinweise: WB: 1:500-1:1000 IHC: 1:50-1:200

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE).

Reinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Buffer: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Lagerung: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-ZA70 (pTyr493) antibody

anti-ZA70 (pTyr493) antibody (Image 2)