P21 Protein (Cdc42/Rac)-Activated Kinase 2 (PAK2) Antikörper

Antigen: P21 Protein (Cdc42/Rac)-Activated Kinase 2 (PAK2)

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Ratte (Rattus)

Applikation: Western Blot (WB), Immunhistochemie (IHC)

Produktnummer: ABIN461191

Produktbeschreibung

Weitere Bezeichnung: PAKgamma

Format: Liquid

Beschreibung: Three isoforms of serine/threonine kinases, designated 7PAK p68, 8PAK p65 and 9PAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste 20, involved in pheromone signaling. The 7, 8, and 9 PAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. There are eight sites of autophosphorylation on 9 PAK, including Ser 19, Ser 141 Thr 402, phosphorylation of Ser 141 and Thr 402 is correlated with 9 PAK activation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates. One such putative substrate is Mek kinase, an upstream effector of Mek4, which is involved in the JNK signaling pathway. While the PAK isoforms interact in a GTP dependent manner with Rac 1 and Cdc 42, they do not interact with Rho.

Spezifität: PAKgamma (R186) pAb detects endogenous levels of PAKgamma protein.

Molekulargewicht: 58.0 kDa

Anwendungen

Applikationshinweise: WB: 1:500-1:1000 IHC: 1:50-1:200

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE).

Reinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Buffer: 1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.

Lagerung: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-P21 Protein (Cdc42/Rac)-Activated Kinase 2 (PAK2) antibody

anti-P21 Protein (Cdc42/Rac)-Activated Kinase 2 (PAK2) antibody (Image 2)