zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antikörper

Antigen: zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70)

Synonyme: SRK, STD, TZK, ZAP-70, FLJ17670, FLJ17679, Srk, mur, 70kDa, mrtle, AI327364, MGC108902, zap70, MGC80670, MGC88962, ZAP70

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Ratte (Rattus)

Applikation: Western Blot (WB), Immunhistochemie (IHC), Immunfluoreszenz (IF)

Produktnummer: ABIN461053

Produktbeschreibung

Weitere Bezeichnung: ZAP-70

Format: Liquid

Beschreibung: The activation of T lymphocytes by antigens is mediated by the T cell receptor (TCR) which is a multisubunit complex assembled from at least six different genes. The TCR subunits include the Ti alpha and beta chains, the CD3 gamma, delta and epsilon chains and a zeta-containing homodimer or heterodimer. The disulfide-linked Ti alpha-beta heterodimer is responsible for antigen recognition, but the short five amino acid cytoplasmic domains of Ti alpha and beta are unlikely to be sufficient to couple to intracellular signaling pathways. In contrast, the structured features of the CD3 and zeta subunits suggest a role in signal transduction. Of these, the zeta chain, which is expressed as either a homodimer or heterodimer, has a short extracellular domain of only nine amino acids, but a larger 113 amino acid cytoplasmic domain. A tyrosine phosphoprotein, ZAP-70, has been identified that associates with zeta and undergoes tyrosine phosphorylation following TCR stimulation.
Synonyms: ZAP70, EC 2.7.10.2, SRK, STD, TZK, ZAP-70

Spezifität: ZAP-70 (S313) pAb detects endogenous levels of ZAP-70 protein.

Molekulargewicht: 70 kDa

Anwendungen

Applikationshinweise: WB: 1:500-1:1000 IHC: 1:50-1:200 IF:1:50-1:200

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE).

Reinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Buffer: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Lagerung: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) antibody

anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) antibody (Image 2)