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Actin-beta Antikörper

Antigen

Actin-beta

Klonalität Polyklonal
Wirt

Kaninchen

Reaktivität

Human, Ratte (Rattus)

Applikation
Western Blot (WB), Immunhistochemie (IHC)
Produktnummer ABIN460395
Menge 100µg  (1 mg/ml)
Preis 572,09 €   Zzgl. Versandkosten €20,00 und MWSt
Lieferung nach
Verfügbarkeit Lieferung in 7 bis 10 Werktagen

Produktbeschreibung

Format Liquid
Beschreibung Beta actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Be aware when using beta actin antibodies as loading controls that although beta actin is normally the best protein to use as a loading control, under some conditions its amounts can vary between samples. In particular, expression of actin in adipose tissue is very low (Spiegelman and Farmer, Cell, 1982, 29(1):53-60) in cells undergoing adipose differentiation actin synthesis decreases by 90%.
Spezifität Actin-beta (D2) pAb detects endogenous levels of Actin-beta protein.
Molekulargewicht 42 kDa

Anwendungen

Applikationshinweise WB: 1:500-1:1000 IHC: 1:50-1:200
Konzentration 1 mg/ml
Reinheit > 95% (by SDS-PAGE).
Reinigung The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Buffer 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Lagerung Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Beschränkungen Nur für Forschungszwecke einsetzbar