M-Cadherin Antikörper

Antigen: M-Cadherin

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Ratte (Rattus)

Applikation: Western Blot (WB), Immunhistochemie (IHC)

Produktnummer: ABIN460386

Produktbeschreibung

Format: Liquid

Beschreibung: Cadherins are a multigene family of Ca++-dependent cell adhesion molecules. They are transmembrane glycoproteins consisting of an extracellular domain, which mediates Ca++-dependent intercellular adhesion by homophilic interactions, a transmembrane region and a cytoplasmic domain. The extracellular domain is divided into a series of subdomains designated EC1-EC5. Homolgies between different members of the cadherin family are most prominent in the cytoplasmic domain and in EC1 and EC2 and much less so in EC5 of the extracellular domain and in the transmembrane region. The binding properties and specificities of the adhesive function are located in the N-terminal part of the molecules. Four members of the cadherin family have been identified and molecularly cloned from mammalian cells. These include the neural (N), epithelial (E), placental (P) and muscle (M) cadherins. M-cadherin is not found in fibroblasts but is expressed at low level in myoblasts and is upregulated following induction of myotube formation, suggesting a specific function in skeletal muscle cell differentiation.
Synonyms: Cadherin-15, Muscle cadherin, M-cadherin, Cadherin-14, CDH15, CDH14, CDH3

Spezifität: M-cadherin (A108) pAb detects endogenous levels of M-cadherin protein.

Molekulargewicht: 91.0 kDa

Anwendungen

Applikationshinweise: WB: 1:500-1:1000 IHC: 1:50-1:200 IF: 1:50-1:200

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE).

Reinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Buffer: 1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.

Lagerung: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-M-Cadherin antibody

anti-M-Cadherin antibody (Image 2)