3 zeta/delta Antikörper

Antigen: 3 zeta/delta

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Ratte (Rattus)

Applikation: Western Blot (WB), Immunhistochemie (IHC), Immunfluoreszenz (IF)

Produktnummer: ABIN460172

Produktbeschreibung

Format: Liquid

Beschreibung: 14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 beta, gamma, epsilon, zeta, eta, theta and sigma. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activities.
Synonyms: YWHAE, 14-3-3E, FLJ45465, KCIP-1, MDCR, MDS

Spezifität: 14-3-3 zeta/delta (W228) pAb detects endogenous levels of 14-3-3 zeta/delta protein.

Molekulargewicht: 28 kDa

Anwendungen

Applikationshinweise: WB: 1:500-1:1000 IHC: 1:50-1:200 IF: 1:50-1:200

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE).

Reinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Buffer: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Lagerung: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-3 zeta/delta antibody

anti-3 zeta/delta antibody (Image 2)