Schaf anti-Maus IgG2a(1a), Allotype Specific Antikörper (HRP)

Antigen: IgG2a(1a), Allotype Specific

Klonalität: Polyklonal

Wirt: Schaf

Reaktivität: Maus

Konjugat: HRP

Applikation: Western Blot (WB), ELISA, Immunhistochemie (IHC), Immunzytochemie (ICC)

Produktnummer: ABIN458495

Produktbeschreibung

Produktmerkmale: Horseradish peroxidase-conjugated IgG fraction of polyclonal sheep antiserum to mouse IgG2a(1a), allotype specific

Immunogen: Highly purified normal homogenous IgG2a(1a) isolated from mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Beschreibung: The reactivity of the antiserum is restricted to the Fc portion of the IgG2a molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against mouse serum of the allelic type Igh-1a, including Balb/C, CBA AKR and NZB, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgG2a(1a). No precipitation reaction is obtained with purified IgG2a(1b) (C57Bl and SJL/J), other IgG subclasses, IgA, IgM, IgG/Fab fragments or other serum proteins. Cross-reactivity The antiserum does not cross-react with any other component of the mouse Ig system. Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants.

Spezifität: The antiserum does not cross-react with any other component of the mouse Ig system. Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. of this antiserum has not been tested in detail, expected chance of cross-reactivity will be small.

Anwendungen

Applikationshinweise: In enzyme-immunocytochemical and immunohistochemical staining for the detection of Igg2a(1a) at the cellular an subcellular level. In non-isotopic assay methodology (e.g. ELISA and Western blotting) to identify and measure IgG2a(1a) in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. Its optimum working dilution should be established by titration. Excess labelled antibody may by itself cause high unspecific background staining or interfere with the specific signal. Working dilutions fro histochemical and cytochemical use are usually between 1:50 and 1:200, in ELISA and comparable non-precipitating antibody-binding assays between 1:100 and 1:1,000. These data should be interpreted as general recommendations only. Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Konzentration: IgG protein concentration 10 mg/ml. Peroxidase/IgG protein molar ratio (E/P) is approximately 1.7. No foreign proteins added. Enzyme marker Horseradish peroxidase enriched for isoenzyme C (RZ=3.2). Manufacturing procedure Conjugation is carried out using

Reinigung: The IgG (7S) fraction is isolated and purified from selected antisera and contains the of the defined antibody specificity. It is free of other serum proteins as tested by immunoelectrophoresis and double radial immuno-diffusion.

Buffer: Horseradish peroxidase-coupled hyperimmune IgG fraction, lyophilized from a solution in phosphate buffered saline (pH7.2). Preservative: No preservative added, as it may interfere with the antibody activity.

Lagerung: Lyophilised at +4°C (10 years), reconstituted at or below -20°C (3-5 years), reconstituted at +4°C at least 7 days. The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, pr olonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile di stilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Pri or to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferab ly used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Beschränkungen: Nur für Forschungszwecke einsetzbar