Kaninchen anti-Maus IgG, IgA and IgM (H&L), Adsorbed For Cross-Reactivity. Antikörper (TRITC)

Antigen: IgG, IgA and IgM (H&L), Adsorbed For Cross-Reactivity.

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Maus

Konjugat: TRITC

Applikation: Immunfluoreszenz (IF)

Produktnummer: ABIN458465

Produktbeschreibung

Produktmerkmale: Tetramethylrhodamine isothiocyanate–conjugated IgG fraction of polyclonal rabbit antiserum to mouse IgG, IgA and IgM, heavy and light chains, adsorbed for human cross-reactivity.

Weitere Bezeichnung: IgG, IgA and IgM (Heavy and Light Chains), Adsorbed For Cross-reactivity.

Immunogen: Purified polyclonal mouse IgG, and homogenous IgA and IgM isolated from mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Beschreibung: The reactivity of the antiserum is directed to the major isotypes of the mouse immunoglobulin system (classes and both light chain types) including antibodies to common determinants, to class and to the surface determinants of the common Fab portion, as tested by immunoelectrophoresis and double radial immunodiffusion against pooled serum and purified immunoglobulins. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal mouse plasma and serum, the characteristic IgG, IgA and IgM precipitin lines are obtained.

Spezifität: Inter-species cross-reactivity is a normal feature of antibodies to mammalian immunoglobulins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentrations of the reactants and the sensitivity of the assay arrangement. If the product is to be used in the presence of material originating from an other species than human, prior screening for cross-reactivity with that material is essential and additional adsorption of the reagent may be required to ensure intra-assay specificity.

Anwendungen

Applikationshinweise: Direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates, to demonstrate immunoglobulins or specific antibodies in cells and tissues, to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen or immune complex using a reference antibody of mouse origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:40. Adsorption: Immunoaffinity adsorbed using insolubilized Ig-depleted mouse serum as required, to eliminate antibodies reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. This product has been passed over appropriate immunoadsorbent to remove antibodies cross-reacting with human immunoglobulins. This renders it specific for use in test systems containing material of human origin (e.g. human tissue/mouse monoclonal antibody to an human tissue constituent/anti mouse Ig isotype specific immunoconjugate).

Konzentration: IgG protein concentration 10 mg/ml. Fluorochrome/IgG protein molar ratio (F/P) is approximately 3.2. No foreign proteins added.

Reinigung: The IgG (7S) fraction is isolated and purified from hyperimmune antisera with strong precipitating activity and contains the bulk of the antibody specificity. It is free of other serum proteins as tested by immunoelectrophoresis and double radial immunodiffusion.

Buffer: TRITC-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). Preservative: No preservative added, as it may interfere with the antibody activity.

Lagerung: Lyophilised at +4°C (10 years), reconstituted at or below -20°C (3-5 years), reconstituted at +4°C at least 7 days. The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile di stilled water. Spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an a liquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Beschränkungen: Nur für Forschungszwecke einsetzbar