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Antikörper
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anti-Intercellular Adhesion Molecule 1 (ICAM1) Antikörper
Intercellular Adhesion Molecule 1 (ICAM1) Antikörper
| Antigen |
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| Synonyme |
BB2, CD54, P3.58, Ly-47, Icam-1, MALA-2, MGC6195, ICAM-1, ICAM1, ICAM |
| Klonalität |
Monoklonal (YN1-1-7-4) |
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Wirt
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Reaktivität
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Human (419), Maus (192), Ratte (Rattus) (116), Rind (Kuh) (60), Schwein (45), Kaninchen (43), Hund (39), Schaf (19), Pferd (16), Huhn (4), Meerschweinchen (2), Hamster (2), Affe (2), Fisch (1)
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Applikation
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 Alternativen Durchflusszytometrie (FACS), Immunpräzipitation (IP), Western Blot (WB), Immunhistochemie (Gefrierschnitte) (IHC (fro)), Funktionale Studien (Func), Immunzytochemie (ICC)
Durchflusszytometrie (FACS) (465), Immunfluoreszenz (IF) (160), Western Blot (WB) (154), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (128), Immunpräzipitation (IP) (114), Immunhistochemie (Paraffinschnitte) (IHC (p)) (85), ELISA (63), Funktionale Studien (Func) (32), Immunhistochemie (IHC) (28), Enzyme Immunoassay (EIA) (24), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (13), Blocking Antibody (Inhibition) (10), Blockierende Antikörper (Inhibition) (10), Immunzytochemie (ICC) (10), Immunoelectron Microscopy (IEM) (7), Immunohistochemistry (Fixed) (IHC (fx)) (5), Intrazelluläre Durchflusszytometrie (ICFC) (4), Dot Blot (Dot) (2), ELISA (Detektionsantikörper) (2)
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| Produktnummer |
ABIN457439 |
| Menge |
0.1 mg (1 mg/ml) (Varianten) |
| Preis |
130,00 € Zzgl. Versandkosten €20,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 3 bis 5 Werktagen |
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Weitere Bezeichnung
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CD54
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Immunogen
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Mouse NS-1 cells
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Format
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Purified
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Isotyp
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IgG2b (Passende Sekundärantikörper)
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Klon
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YN1-1-7-4
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Beschreibung
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CD54 (ICAM-1) is a member of the C2 subset of immunoglobulin superfamily. It is atransmembrane molecule with 7 potential N-glycosylated sites, expressed on restingmonocytes and endothelial cells and can be upregulated on many other cells, e.g. withlymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also onkeratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion bybinding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction ofCD54 with LFA-1 enhances antigen-specific T-cell activation.
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Spezifität
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The rat monoclonal antibody YN1/1.7.4 reacts with CD54 (ICAM-1), a 85-110 kDa type Itransmembrane glycoprotein expressed on activated endothelial cells, T lymphocytes, Blymphocytes, monocytes, macrophages, granulocytes and dendritic cells, the expression ofCD54 is upregulated by activation.
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Applikationshinweise
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Flow Cytometry: Recommended dilution: 1 µg/ml. Application note: non-reducing conditionsImmunohistochemistry (frozen sections). Application note: acetone fixation. Functional Application: Blocking
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Konzentration
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1 mg/ml
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Reinheit
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> 95% (by SDS-PAGE)
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Reinigung
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Purified from hybridoma culture supernatant by protein-G affinity chromatography.
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Buffer
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Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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Lagerung
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Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.
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Forschungsgebiet
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CD Antigene, Oberflächenrezeptoren der Immunzellen , Immunologie, Inflammation, Infektionen
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Alternativen zu Antigen "Intercellular Adhesion Molecule 1 (ICAM1)", Typ "Antikörper" finden
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Wirte
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Maus (284), Kaninchen (194), Ratte (98), Hamster (8), Schaf (1)
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Reaktivitäten
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Human (419), Maus (192), Ratte (Rattus) (116), Rind (Kuh) (60), Schwein (45), Kaninchen (43), Hund (39), Schaf (19), Pferd (16), Huhn (4), Meerschweinchen (2), Hamster (2), Affe (2), Fisch (1)
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Applikationen
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Durchflusszytometrie (FACS) (465), Immunfluoreszenz (IF) (160), Western Blot (WB) (154), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (128), Immunpräzipitation (IP) (114), Immunhistochemie (Paraffinschnitte) (IHC (p)) (85), ELISA (63), Funktionale Studien (Func) (32), Immunhistochemie (IHC) (28), Enzyme Immunoassay (EIA) (24), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (13), Blocking Antibody (Inhibition) (10), Blockierende Antikörper (Inhibition) (10), Immunzytochemie (ICC) (10), Immunoelectron Microscopy (IEM) (7), Immunohistochemistry (Fixed) (IHC (fx)) (5), Intrazelluläre Durchflusszytometrie (ICFC) (4), Dot Blot (Dot) (2), ELISA (Detektionsantikörper) (2)
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Konjugate
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Biotin (60), FITC (54), PE (50), Alexa Fluor 488 (14), Alexa Fluor 647 (11), PE,Cy5 (9), APC (8), Alexa Fluor 350 (7), Alexa Fluor 555 (7), Cy3 (7), Cy5 (7), Cy5.5 (7), Cy7 (7), Gold (7), HRP (7), PE,Cy3 (7), PE,Cy5.5 (7), PE,Cy7 (7), RBITC (7), RPE (7), Alexa Flour 488 (6), Pacific Blue (3), Alexa Fluor 700 (2), Alexa Fluor 546 (1), PerCP (1)
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Epitope
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pTyr512 (9), C-Term (3), AA 253-267 (1), Domain D1 (1)
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