HSF1 Antikörper

Produktnummer ABIN453930

Produktdetails anti-HSF1 Antikörper

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Kaninchen, Affe, Meerschweinchen, Hamster

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser HSF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Electrophoretic Mobility-Shift Assay (EMSA)

Spezifität: This antibody detects an ~85 kDa protein in unstressed cell lysates, and an ~95 kDa protein in heat shocked cell lysates, corresponding to the molecular mass of inactive and active forms of HSF1 on SDS-PAGE immunoblots.

Kreuzreaktivität (Details): Species reactivity (tested):Human, Mouse, Rat, Bovine, Guinea pig, Hamster, Monkey, Rabbit

Produktmerkmale: Synonyms: HSTF1, HSF-1, Heat shock transcription factor 1

Aufreinigung: Purified

Immunogen: Purified recombinant HSF1 protein

Klon: 10H8

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western blot (see ref.1). Immunoprecipitation. Immunocytochemistry (see ref.1). ELISA (see ref.1). Gel Mobility Shift Assay (see ref.1).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.09 % sodium azide in 50 % glycerol

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C. Avoid repeated freezing andthawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu HSF1

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Andere Bezeichnung: Heat Shock Factor 1 / HSF1 (HSF1 Produkte)

Synonyme: hsf1 antikoerper, Hsf antikoerper, xhsf1 antikoerper, AA960185 antikoerper, HSTF1 antikoerper, HSF1 antikoerper, HSF antikoerper, HSF1c antikoerper, hsf1a antikoerper, hsf1b antikoerper, zHSF1a antikoerper, zHSF1b antikoerper, hstf1 antikoerper, heat shock factor antikoerper, Heat Shock Factor antikoerper, heat shock factor 1 antikoerper, heat shock transcription factor 1 antikoerper, heat shock transcription factor 1 S homeolog antikoerper, hsf1 antikoerper, hsf-1 antikoerper, Hsf1 antikoerper, HSF1 antikoerper, hsf1.S antikoerper

Hintergrund: HSF1, or heat shock factor 1, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the Nterminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transciptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11).Synonyms: HSF-1, HSTF1, Heat shock transcription factor 1

Gen-ID: 3297

UniProt: Q00613