Adeno-Associated Virus 2 (AAV-2) (intact particle) Antikörper (Biotin)

Antigen: Adeno-Associated Virus 2 (AAV-2) (intact particle)

Klonalität: Monoklonal (A20)

Wirt: Maus

Konjugat: Biotin

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Enzyme Immunoassay (EIA), Immunfluoreszenz (IF)

Produktnummer: ABIN453888

Produktbeschreibung

Produktmerkmale: Synonyms: AAV2

Immunogen: Adeno-associated virus capsid proteins and virus particles

Isotyp: IgG3

Klon: A20

Spezifität: A20 specifically reacts with intact adeno-associated virus particles, empty and fullcapsids. Recognizes a conformational epitope of assembled capsids, not present indenatured capsid proteins and native but unassembled capsid proteins. Species: Human, Monkey. Others not tested.

Anwendungen

Applikationshinweise: Immunofluorescence: Overnight at 2-8°C. Immunohistochemistry: Overnight at 2-8°C. ELISA: 1/20, use PBS with 0.05% Tween 20, 1 h at 37°C. The antibody cannot be used for immunoblotting. Epitope mapping experiments (Wobus et al. , see below) identified four immunoreactive(discontinous) regions. The major reaction was attributed to sequence aa 369 to aa378 ofAAV-2 capsids. The antibody is also useful for neutralizing experiments. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 20 µg/ml

Lagerung: After reconstitution store the antibody at 2 - 8°C. Shelf life: 6 weeks from despatch.

Beschränkungen: Nur für Forschungszwecke einsetzbar