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Rekombinanter RFP Antikörper (AZDye 568)

RFP Reaktivität: Discosoma IF, ICC, FACS Wirt: Alpaca Monoclonal 2B12 AZDye 568 single-domain Antibody (sdAb) Recombinant Antibody
Produktnummer ABIN4368331
  • Target Alle RFP Antikörper anzeigen
    RFP (Red Fluorescent Protein (RFP))
    Antikörpertyp
    Recombinant Antibody
    Fragment
    single-domain Antibody (sdAb)
    Reaktivität
    • 52
    • 1
    Discosoma
    Wirt
    • 23
    • 15
    • 8
    • 5
    • 3
    Alpaca
    Expressionssystem
    E.coli
    Klonalität
    • 31
    • 23
    Monoklonal
    Konjugat
    • 21
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser RFP Antikörper ist konjugiert mit AZDye 568
    Applikation
    • 29
    • 21
    • 15
    • 11
    • 9
    • 8
    • 6
    • 6
    • 2
    • 1
    • 1
    • 1
    Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
    Verwendungszweck
    Camelid sdAb anti-RFP conjugated with AZDye 568 (Alexa Fluor 568 equivalent), Clone 2B12
    Spezifität
    Recognizes most common red fluorescent proteins like mRFP and derivatives like mCherry, mScarlet-i, tdTomato, dsRed and mOrange.
    Kreuzreaktivität (Details)
    Does not cross-react with GFP or mTagBFP derivatives.
    Produktmerkmale
    A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.
    Aufreinigung
    Produced in: E.coli
    Immunogen
    Recombinant protein corresponding to AA 1 to 225 from sea anemone RFP (UniProt Id: Q9U6Y8)
    Klon
    2B12
    Labeling Ratio
    a single fluorophore is coupled to exactly one sdAb
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  • Applikationshinweise
    Recommended dilution 1:500
    Kommentare

    Each fluorophore is coupled to exactly one sdAb, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95% guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Add 200 µl H2O for reconstitution. Possibly add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
    Reconstitute immediately upon receipt!
    Konzentration
    5 μM
    Buffer
    5 μM fluorescently labeled sdAb in buffered saline, 50 % glycerol, 0.09 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
    Lagerung
    -20 °C,-80 °C
    Informationen zur Lagerung
    Up to 3 months store at -20 °C. Up to 12 months store at -80 °C or below
    Haltbarkeit
    12 months
  • Target
    RFP (Red Fluorescent Protein (RFP))
    Andere Bezeichnung
    RFP (RFP Produkte)
    Molekulargewicht
    26 kDa
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