SMAD3 Antikörper (SMAD, Mothers Against DPP Homolog 3) (pSer423)

Details for Product anti-SMAD3 Antibody No. ABIN4354683, Anbieter: Anmelden zum Anzeigen
Antigen
  • madh3
  • madh3a
  • smad3
  • wu:fa99e03
  • HSPC193
  • HsT17436
  • JV15-2
  • LDS1C
  • LDS3
  • MADH3
  • XSmad3
  • XenMLP
  • Xmad3
  • madh3-A
  • Madh3
  • AU022421
  • Smad 3
  • mad3
  • SMAD family member 3
  • MAD homolog 3a (Drosophila)
  • mothers against decapentaplegic homolog 3
  • SMAD3
  • smad3a
  • LOC100346303
  • smad3
  • Smad3
Epitop
pSer423, pSer425
56
51
50
41
35
23
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16
13
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8
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3
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3
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2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Huhn, Rind (Kuh), Human, Maus, Schwein, Ratte (Rattus), Xenopus laevis, Zebrafisch
533
283
264
37
28
23
22
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18
16
12
4
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3
2
2
1
1
1
1
1
1
Wirt
Kaninchen
468
58
23
Klonalität
Polyklonal
Konjugat
Dieser SMAD3 Antikörper ist unkonjugiert
20
19
17
9
9
9
8
8
8
8
8
5
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5
3
3
1
1
1
1
1
1
1
1
1
1
1
Applikation
ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
386
223
202
107
105
67
45
36
26
18
9
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7
2
2
2
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 417-425 of human SMAD3 protein.
Spezifität This antibody is directed against the phosphorylated form of human Smad3 protein at the pS423 and pS425 residues.
Reinigung Immunogen affinity purified
Andere Bezeichnung Smad3 (SMAD3 Antibody Abstract)
Hintergrund Gene Symbol: SMAD3
Gen-ID 4088
NCBI Accession NC_000015
Forschungsgebiet Signaling, Transcription Factors, Cancer
Pathways Zellzyklus, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagie
Applikationshinweise Western Blot 1:2000-1:20000, Flow Cytometry 1:1:10 - 1:1000, ELISA 1:15000 - 1:30000, Immunohistochemistry 1:500, Immunohistochemistry-Paraffin 1:10-1:500Expect a band approximately 48 kDa in size corresponding - phosphorylated Smad3 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract. Less than 0.2 % reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for dual phosphorylated pS423 and pS425 of Smad3. Stimulation with 2 ng/mL TGF-beta for 1 hour is suggested.
Kommentare

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Buffer contains: 0.01 % Sodium Azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -80 °C,-20 °C
Informationen zur Lagerung Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Bilder des Herstellers
Western Blotting (WB) image for anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (pSer423), (pSer425) antibody (ABIN4354683) Western Blot: Smad3 [p Ser423, p Ser425] Antibody [NBP1-77836] - Shows detection of e...
Immunohistochemistry (IHC) image for anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (pSer423), (pSer425) antibody (ABIN4354683) Immunohistochemistry: Smad3 [p Ser423, p Ser425] Antibody [NBP1-77836] - Used at 2.5 ...
Produkt verwendet in: Rogers, Moreno, Smith, Husson, Bukanov, Ledbetter, Budman, Lu, Wang, Ibraghimov-Beskrovnaya, Natoli: "Differences in the timing and magnitude of Pkd1 gene deletion determine the severity of polycystic kidney disease in an orthologous mouse model of ADPKD." in: Physiological reports, Vol. 4, Issue 12, 2016 (PubMed).

Yamashita, Fatyol, Jin, Wang, Liu, Zhang: "TRAF6 mediates Smad-independent activation of JNK and p38 by TGF-beta." in: Molecular cell, Vol. 31, Issue 6, pp. 918-24, 2008 (PubMed).

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