TLR4 Antikörper

Antigen: TLR4

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Western Blot (WB)

Produktnummer: ABIN411611

Produktbeschreibung

Produktmerkmale: 100 ug affinity purified rabbit anti-TLR4 polyclonal antibody. Reacts with human, mouse, rat. Tested in Western blotting. Other applications have not been determined.

Gen-ID: 29260

Immunogen: Synthetic peptide corresponding to residues surrounding amino acids 819 of rat TLR4

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: TLR4 (Toll-like receptor 4) is a mammalain homologue of the Drosophila toll protein. The human TLR4 is a type-1 transmembrane protein containing 799 amino acid residues. The extracellular domain has leucine-rich repeats and the intracellular domain is similar to that of IL-1 receptor. TLR4 is a receptor for lipopolysaccharide (LPS). In mice that are genetically tolerant to endotoxin, mutations have been identified in the intracellular domain of TLR4 that abolish LPS responsiveness. TLR4 uses a mechanism similar to that of the IL-1 receptor for signal transduction that leads to activation of NF-kB.

Anwendungen

Applikationshinweise: Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~92 kDa TLR4 of rat origin. Reactivity to other species has not been tested.

Konzentration: 0.5 mg/ml

Reinigung: Affinity Purified Antibody

Buffer: 100 µg (0.5 mg/ml) affinity purified rabbit anti-TLR4 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

Lagerung: Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.

Forschungsgebiet: Immunologie, Angeborene Immunität, Adaptive Immunität, Artherosklerose, Signaltransduktion, Inflammation

Beschränkungen: Nur für Forschungszwecke einsetzbar