Signal Transducer and Activator of Transcription 1 (STAT1) Antikörper

Antigen: Signal Transducer and Activator of Transcription 1 (STAT1)

Synonyme: ISGF-3, STAT91, DKFZp686B04100, XStat1, STAT1, STAT4, isgf-3, stat91, MGC147452, DKFZp459D206

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Western Blot (WB)

Produktnummer: ABIN411594

Produktbeschreibung

Produktmerkmale: 100 ug affinity purified rabbit anti-Stat1 polyclonal antibody. Detects both alpha (91 kDa) and beta (84 kDa) subunits of STAT1. Reacts with human/mouse/rat. Works well in Western blot. Other applications have not been determined. Blocking peptide (Cat.# 3133RBP-50) is available separately.

Weitere Bezeichnung: Stat1

Gen-ID: 25124

Immunogen: Synthetic peptide surrounding amino acid 672 of rat Stat1

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: STATs (signal transducers and activators of transcription) are a family of cytoplamic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signalling peptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes. Among the seven STATs (Stat1, Stat2, Stat3, Stat4, Stat5a, Stat5b, and Stat6). Stat1, Stat3, Stat5a and Stat5b have wide activation profiles. Stat1 is activated by many different ligands including IFN family (IFN-a, IFN-b, IFN-r, and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF-1), and receptor tyrosine kinases (EGF, PDGF, and CSF-1). Stat1 has two forms, the 91 kDa STAT1α and the 84 kDa STAT1β, which are coded by the same gene with splicing variant.

Spezifität: The antibody recognizes both alpha (91 kDa) and beta (84 kDa) subunits of STAT1 in samples from human, mouse, and rat origins. Other species have not been determined.

Anwendungen

Applikationshinweise: Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes both alpha (91 kDa) and beta (84 kDa) subunits of STAT1 in samples from human, mouse, and rat origins.

Konzentration: 0.5 mg/ml

Reinigung: Affinity Purified Antibody

Buffer: 100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.

Lagerung: Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.

Beschränkungen: Nur für Forschungszwecke einsetzbar