PUMA Bbc3 (0) Antikörper

Antigen: PUMA Bbc3 (0)

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Western Blot (WB)

Produktnummer: ABIN411574

Produktbeschreibung

Produktmerkmale: 100 ug affinity purified rabbit anti-PUMA/bbc3 polyclonal antibody. Reacts with human, mouse and rat. Detects PUMA alpha (193 a.a., ~25 kDa) and beta (131 a.a., ~16 kDa) forms in Western blotting. Other applications have not been determined. Blocking peptide (Cat.# 3393RBP-50) is available separately.

Weitere Bezeichnung: PUMA/bbc3

Gen-ID: 27113

Immunogen: Synthetic peptide surrounding amino acid 177 of human PUMA

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: PUMA (p53 upregulated modulator of apoptosis) is one of the pro-apoptotic Bcl-2 family members which are also transcriptional targets of p53. PUMA gene encodes two BH3 domain-containing proteins termed PUMA-α and PUMA-β. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

Spezifität: The antibody detects ~25 kDa (193 a.a.) PUMA alpha and ~16 kDa (131 a.a.) PUMA beta in samples from human, mouse and rat origins.

Anwendungen

Applikationshinweise: Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects ~25 kDa (193 a.a.) PUMA alpha and ~16 kDa (131 a.a.) PUMA beta in samples from human, mouse and rat origins.

Konzentration: 0.5 mg/ml

Reinigung: Affinity Purified Antibody

Buffer: 100 µg (0.5 mg/ml) affinity purified rabbit anti-PUMA polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

Lagerung: Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.

Beschränkungen: Nur für Forschungszwecke einsetzbar