Pim-1 Oncogene (PIM1) Antikörper

Antigen: Pim-1 Oncogene (PIM1)

Synonyme: PIM, Pim-1, pim2, cb1068, fj47b05, wu:fj47b05, pim1-a, PIM1, pim3, MGC89574, pim-1, PAP-1

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN411562

Produktbeschreibung

Produktmerkmale: 100 ug affinity purified rabbit anti-PIM1 polyclonal antibody. Reacts with human, mouse and rat. Detects the 33 kDa PIM1 isoform in Western Blot analysis. Jurkat cell lysate can be used as a positive control. Blocking peptide (Cat.# 3787RBP-50) is available separately.

Weitere Bezeichnung: PIM1

Gen-ID: 24649

Immunogen: Synthetic peptide surrounding amino acid 296 of rat PIM1

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: The proto-oncogene Pim1 belongs to a family of serine/threonine protein kinases that are highly conserved through evolution in multicellular organisms. Pim1 is involved in the control of cytokine-mediated cell proliferation, differentiation and survival of lymphoid and myeloid cells as well as others. Expression of Pim1 can be stimulated by a variety of growth factors and is regulated at four different levels: transcriptional, post-transcriptional, translational and post-translational. Recently, Pim1 has been shown to enhance the activities of p100, c-Myb and Cdc 25a and in part this might explain reported effects of Pim1 on mitogenesis.

Anwendungen

Applikationshinweise: Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~33 kDa of PIM1 isoform from samples of human, mouse and rat origins. Reactivity to other species has not been determined.

Konzentration: 0.5 mg/ml

Reinigung: Affinity Purified Antibody

Buffer: 100 µg (0.5 mg/ml) affinity purified rabbit anti-PIM1 polyclonal antibody in phosphate (PBS, pH 7.2) containing 30% glycerol, 0.5 % BSA and 0.01% thimerosal.

Lagerung: Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.

Forschungsgebiet: Krebs, Hämopoetische Vorläufer

Beschränkungen: Nur für Forschungszwecke einsetzbar